Genotyping and phylogenetic location of one clinical isolate of Bacillus anthracis isolated from a human in Russia

Autor:	A. N. Kulichenko, T. M. Golovinskaya, S. V. Pisarenko, E. I. Eremenko, Dmitry A. Kovalev, Dmitriy K. Tchmerenko, Lyudmila Yu. Aksenova, Anna Yu. Evchenko, Irina V. Kuznetsova, N. P. Buravtseva, Vitaliy Yu. Morozov, Olga V. Semenova, Olga V. Bobrisheva, A. G. Ryazanova
Rok vydání:	2019
Předmět:	Microbiology (medical) Genotype Population lcsh:QR1-502 Minisatellite Repeats Multiple Loci VNTR Analysis Microbiology Polymorphism Single Nucleotide lcsh:Microbiology Russia Anthrax 03 medical and health sciences Whole-genome single-nucleotide-polymorphism analysis (wgSNP) Single nucleotide polymorphism (SNP) Humans education Genotyping Phylogeny Comparative genomics Genetics Whole genome sequencing 0303 health sciences education.field_of_study Molecular Epidemiology biology Phylogenetic tree Whole Genome Sequencing 030306 microbiology Whole genome sequencing (WGS) biology.organism_classification Bacillus anthracis Phylogeography GenBank MLVA) Multiple locus variable number of tandem repeat analysis (VNTR Genome Bacterial Research Article
Zdroj:	BMC Microbiology BMC Microbiology, Vol 19, Iss 1, Pp 1-9 (2019)
ISSN:	1471-2180
Popis:	Background Anthrax is a zoonotic disease caused by the Gram-positive bacterium Bacillus anthracis. In Russia, there are more than 35 thousand anthrax stationary unfavourable sites. At the same time, there is very little published information about the isolates of B. anthracis from the territory of Russia. In this study, we report the use of whole genome sequencing (WGS) and bioinformatics analysis to characterize B. anthracis 81/1 strain isolated in Russia in 1969 from a person during an outbreak of the disease in the Stavropol region. Results We used 232 B. anthracis genomes, which are currently available in the GenBank database, to determine the place of the Russian isolate in the global phylogeny of B. anthracis. The studied strain was characterized by PCR-based genetic methods, such as Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA), canonical single nucleotide polymorphisms (canSNP), as well as the method of full-genomic analysis of nucleotide polymorphisms (wgSNP). The results indicate that the Russian B. anthracis 81/1 strain belongs to Trans-Eurasion (TEA) group, the most representative in the world. Conclusions In this study, the full genomic sequence of virulent B. anthracis strain from Russia was characterized for the first time. As a result of complex phylogenetic analysis, the place of this isolate was determined in the global phylogenetic structure of the B. anthracis population, expanding our knowledge of anthrax phylogeography in Russia. Electronic supplementary material The online version of this article (10.1186/s12866-019-1542-3) contains supplementary material, which is available to authorized users.
Databáze:	OpenAIRE
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