Detection of cytoplasmic CD antigens within normal human peripheral blood leucocytes
| Autor: | Elizabeth Loudon, Roderick K. Ferrier, Carol Campbell, G. P. Sandilands, Martina E. Rodie, Andrew G. Marsh, Anna Gondowidjojo, Birgit Hauffe |
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| Rok vydání: | 2003 |
| Předmět: |
Cytoplasm
Neutrophils Sialic Acid Binding Ig-like Lectin 2 Immunology Antigen presentation chemical and pharmacologic phenomena Major histocompatibility complex Polymerase Chain Reaction MHC class II antigen Immune system Antigen Antigens CD Lectins Humans Immunology and Allergy RNA Messenger In Situ Hybridization CD antigen Antigen Presentation biology Antigen processing Original Articles Flow Cytometry Molecular biology Antigens Differentiation B-Lymphocyte biology.protein Cell Adhesion Molecules CD80 |
| Zdroj: | Immunology. 108:329-337 |
| ISSN: | 1365-2567 0019-2805 |
| DOI: | 10.1046/j.1365-2567.2003.01591.x |
| Popis: | Polymorphonuclear neutrophils (PMNs) are capable of synthesizing various pro-inflammatory cytokines which may indirectly influence specific immune responses. PMNs may also have the capacity to present foreign peptides to helper T cells (Th cells). In support of this hypothesis, recent studies have shown that neutrophils, when activated by the correct combination of cytokines, can be induced to express cell surface major histocompatibility complex (MHC) Class II (DR) antigen, CD80 (B7.1) and CD86 (B7.2): molecules required for antigen presentation and subsequent T-cell activation. In this study we have used normal ‘resting’ human peripheral blood neutrophils and demonstrated, using a mild fixation and permeabilization protocol, significant cytoplasmic ‘stores’ of these molecules known to be important in antigen presentation. Cytoplasmic MHC Class II antigen was found with two out of 20 normal donors tested whereas cytoplasmic CD80 and CD86 were found to a variable extent within all normal donors. Surprisingly, we also found several other neutrophil cytoplasmic CD antigens more commonly associated with B cells, i.e. CD20, CD21 (CR2/EBV-R) and CD22 (BL-CAM). All of these antigens were confined to the ‘resting’ cell cytoplasm and were never found to be expressed on the cell surface. To exclude the possibility that these antigens were absorbed from plasma and to provide evidence for active synthesis, we used a novel whole blood in situ hybridization flow cytometry assay method to detect mRNA specific for these antigens within normal PMNs. We also conducted real-time polymerase chain reactions to confirm these findings using CD22 as a good example of an ‘inappropriately expressed’ CD antigen. These observations therefore provide support for the hypothesis that human PMNs have the potential to express molecules required for antigen presentation and cell signalling. |
| Databáze: | OpenAIRE |
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