A polypyrrole protein microarray for antibody–antigen interaction studies using a label-free detection process

Autor: Ludivine Grosjean, Andre Roget, Marie-Bernadette Villiers, Emilie Mercey, Thierry Livache, Boutheina Cherif, Patrice N. Marche, Yves Levy
Přispěvatelé: Structures et propriétés d'architectures moléculaire (SPRAM - UMR 5819), Institut Nanosciences et Cryogénie (INAC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Contrôle moléculaire de la réponse immune specifique, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Charles Fabry de l'Institut d'Optique / Macsybio, Laboratoire Charles Fabry de l'Institut d'Optique (LCFIO), Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS), Ludivine Grosjean was supported by a grant from Genoptics (Orsay, France) and Boutheina Cherif was supported by the 'Ministère de l'Enseignement Supérieur de Tunisie' and the 'CEA, Direction des Relations Internationales.', Marche, Patrice, Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Institut Nanosciences et Cryogénie (INAC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11)
Rok vydání: 2005
Předmět:
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Polymers
MESH: Chemistry
Analytical
02 engineering and technology
Polypyrrole
Chorionic Gonadotropin
01 natural sciences
Biochemistry
Antigen-Antibody Reactions
chemistry.chemical_compound
Surface plasmon resonance imaging
MESH: Antigen-Antibody Reactions
MESH: Animals
[INFO.INFO-BT]Computer Science [cs]/Biotechnology
chemistry.chemical_classification
MESH: Protein Array Analysis
021001 nanoscience & nanotechnology
MESH: Polymers
MESH: Surface Plasmon Resonance
Interaction studies
Antigen
Scientific method
MESH: Pyrroles
Protein microarray
[SDV.IMM]Life Sciences [q-bio]/Immunology
0210 nano-technology
[SDV.IMM] Life Sciences [q-bio]/Immunology
Surface Properties
Protein Array Analysis
Biophysics
Nanotechnology
In Vitro Techniques
Chemistry Techniques
Analytical
MESH: Chorionic Gonadotropin
Animals
Humans
Molecule
Pyrroles
Molecular Biology
Antibody
Label free
MESH: Surface Properties
MESH: Humans
Biomolecule
010401 analytical chemistry
Cell Biology
Surface Plasmon Resonance
[SDV.BIO] Life Sciences [q-bio]/Biotechnology
0104 chemical sciences
[INFO.INFO-BT] Computer Science [cs]/Biotechnology
chemistry
MESH: Muramidase
Muramidase
Zdroj: Analytical Biochemistry
Analytical Biochemistry, 2005, 347 (2), pp.193-200. ⟨10.1016/j.ab.2005.09.033⟩
Analytical Biochemistry, Elsevier Masson, 2005, 347 (2), pp.193-200. ⟨10.1016/j.ab.2005.09.033⟩
ISSN: 0003-2697
1096-0309
DOI: 10.1016/j.ab.2005.09.033
Popis: International audience; Protein microarray is a promising technology that should combine rapidity and easy use with high throughput and versatility. This article describes a method in which an electrocopolymerization process is employed to graft biological molecules on to a chip so that surface plasmon resonance imaging may be used to detect molecular interactions. Copolymerization of pyrrole-modified protein and pyrrole is an efficient grafting process which immobilizes molecules at defined positions on a gold surface. Surface plasmon resonance imaging is an optical technique that allows real-time simultaneous detection of molecular interactions on a large number of spots without labeling. This method was successfully used to analyze antibody-antigen interactions. This illustrates its high specificity and good sensitivity and demonstrates its suitability for biological studies.
Databáze: OpenAIRE
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