An antigenic peptide produced by peptide splicing in the proteasome
| Autor: | Sandra Morel, Gérard Degiovanni, Pierre van der Bruggen, Thierry Boon, Jacques Chapiro, Vincent Stroobant, Nathalie Vigneron, Benoît Van den Eynde, Annie Ooms |
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| Rok vydání: | 2004 |
| Předmět: |
Proteasome Endopeptidase Complex
Antigen presentation Peptide Biology Major histocompatibility complex Transfection Interferon-gamma Antigen Protein splicing Multienzyme Complexes Cell Line Tumor Animals Humans Protein Splicing Protein Precursors Melanoma chemistry.chemical_classification Antigen Presentation Multidisciplinary Membrane Glycoproteins HLA-A Antigens Peptide Fragments Amino acid Neoplasm Proteins Cysteine Endopeptidases Electroporation Proteasome chemistry Biochemistry Models Chemical RNA splicing COS Cells biology.protein T-Lymphocytes Cytotoxic gp100 Melanoma Antigen |
| Zdroj: | Science (New York, N.Y.). 304(5670) |
| ISSN: | 1095-9203 |
| Popis: | CD8 T lymphocytes recognize peptides of 8 to 10 amino acids presented by class I molecules of the major histocompatibility complex. Here, CD8 T lymphocytes were found to recognize a nonameric peptide on melanoma cells that comprises two noncontiguous segments of melanocytic glycoprotein gp100PMEL17. The production of this peptide involves the excision of four amino acids and splicing of the fragments. This process was reproduced in vitro by incubating a precursor peptide of 13 amino acids with highly purified proteasomes. Splicing appears to occur by transpeptidation involving an acyl-enzyme intermediate. Our results reveal an unanticipated aspect of the proteasome function of producing antigenic peptides. |
| Databáze: | OpenAIRE |
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