In vivo upregulation of nitric oxide synthases in healthy rats
| Autor: | Heng Wu, Keith A. Webster, Jaqueline Arias, Ying Jin, Jorge Bassuk, Arkady Uryash, Jose A. Adams, Paul Kurlansky |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Nitric Oxide Synthase Type III Proteome Physiology Clinical Biochemistry Ischemia Blood Pressure Nitric Oxide Synthase Type I Nitric Oxide Biochemistry Article Nitric oxide Rats Sprague-Dawley Electrolytes chemistry.chemical_compound Downregulation and upregulation Enos In vivo Internal medicine medicine Animals Phosphorylation Extracellular Signal-Regulated MAP Kinases Protein kinase B Cardioprotection Analysis of Variance biology Myocardium biology.organism_classification medicine.disease Immunohistochemistry Rats Up-Regulation Endocrinology chemistry Stress Mechanical Blood Gas Analysis Proto-Oncogene Proteins c-akt Reperfusion injury Signal Transduction |
| Zdroj: | Nitric Oxide. 21:63-68 |
| ISSN: | 1089-8603 |
| DOI: | 10.1016/j.niox.2009.05.004 |
| Popis: | Periodic acceleration (pGz), sinusoidal motion of the whole body in a head-foot direction in the spinal axis, is a novel noninvasive means for cardiopulmonary support and induction of pulsatile shear stress. pGz increases plasma nitrite levels, in vivo and in vitro. Additionally, pGz confers cardioprotection in models of ischemia reperfusion injury. We hypothesize that pGz may also confer a cardiac phenotypic change by upregulation of the expression of the various NO synthase (NOS) isoforms in vivo. pGz was applied for 1h to awake restrained male rats at 2 frequencies (360 and 600 cpm) and acceleration (Gz) of +/-3.4 m/s(2). pGz did not affect arterial blood gases or electrolytes. pGz significantly increased total nitrosylated protein levels, indicating increased NO production. pGz also increased mRNA and protein levels of eNOS and nNOS, and phosphorylated eNOS in heart. pGz increased Akt phosphorylation (p-AKT), but not total Akt, or phosphorylated ERK1/2. Inducible (i) NOS levels were undetectable with or without pGz. Immunoblotting revealed the localization of nNOS, exclusively in cardiomyocyte, and pGz increased its expression. We have demonstrated that pGz changes myocardial NOS phenotypes. Such upregulation of eNOS and nNOS was still evident 24h after pGz. Further studies are needed to understand the biochemical and biomechanical signal transduction pathway for the observed NOS phenotype changed induced by pGz. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect