Alterations in the phenylpropanoid pathway affect poplar ability for ectomycorrhizal colonisation and susceptibility to root-knot nematodes

Autor: Geert Goeminne, Mondher El Jaziri, Marie Baucher, Rebecca Van Acker, Marc Behr, Janice de Almeida Engler, Gilles Pilate, Wout Boerjan, Claire Veneault-Fourrey, Annegret Kohler, Fabien Baldacci-Cresp, Kris Morreel, Adeline Mol
Přispěvatelé: Laboratoire de Biotechnologie Végétale, Université libre de Bruxelles (ULB), Interactions Arbres-Microorganismes (IAM), Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Center for Plant Systems Biology (PSB Center), Vlaams Instituut voor Biotechnologie [Ghent, Belgique] (VIB), Department of Plant Biotechnology and Bioinformatics, Universiteit Gent = Ghent University [Belgium] (UGENT), Biologie intégrée pour la valorisation de la diversité des Arbres et de la Forêt (BioForA), Office National des Forêts (ONF)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Fonds de la Recherche Scientifique - FNRS T. 0004.14, Multidisciplinary Research Partnership Biotechnology for a sustainable economy, European Commission's Directorate-General for Research within the 7th Framework Program (FP7/2007-2013) 270,089, ANR-11_LABX-0002-01 CFP15, United States Department of Energy (DOE)
Rok vydání: 2020
Předmět:
0106 biological sciences
Nematoda
Cinnamyl-alcohol dehydrogenase
Plant Science
Fungus
Lignin
Transgenic poplar
01 natural sciences
Secondarymetabolites
03 medical and health sciences
chemistry.chemical_compound
Symbiosis
Gene Expression Regulation
Plant
Mycorrhizae
Botany
Genetics
medicine
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Animals
Root-knot nematode
Monolignol
Transcriptomics
Molecular Biology
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
2. Zero hunger
0303 health sciences
biology
Phenylpropanoid
fungi
food and beverages
General Medicine
Ectomycorrhiza . Monolignol . Root-knot nematode . Secondary metabolites . Transcriptomics . Transgenic poplar
15. Life on land
biology.organism_classification
medicine.disease
Physiologie des plantes vasculaires
Mycologie
Ectomycorrhiza
Colonisation
Populus
Nematode infection
chemistry
010606 plant biology & botany
Zdroj: Mycorrhiza, 30
Mycorrhiza
Mycorrhiza, Springer Verlag, 2020, 30 (5), pp.555-566. ⟨10.1007/s00572-020-00976-6⟩
ISSN: 1432-1890
0940-6360
DOI: 10.1007/s00572-020-00976-6
Popis: This study investigates the impact of the alteration of the monolignol biosynthesis pathway on the establishment of thein vitro interaction of poplar roots either with a mutualistic ectomycorrhizal fungus or with a pathogenic root-knotnematode. Overall, the five studied transgenic lines downregulated for caffeoyl-CoA O-methyltransferase(CCoAOMT), caffeic acid O-methyltransferase (COMT), cinnamoyl-CoA reductase (CCR), cinnamyl alcoholdehydrogenase (CAD) or both COMT and CAD displayed a lower mycorrhizal colonisation percentage, indicating alower ability for establishing mutualistic interaction than the wild-type. The susceptibility to root-knot nematode infectionwas variable in the five lines, and the CAD-deficient line was found to be less susceptible than the wild-type. Wediscuss these phenotypic differences in the light of the large shifts in the metabolic profile and gene expression patternoccurring between roots of the CAD-deficient line and wild-type. A role of genes related to trehalose metabolism,phytohormones, and cell wall construction in the different mycorrhizal symbiosis efficiency and nematode sensitivitybetween these two lines is suggested. Overall, these results show that the alteration of plant metabolism caused by therepression of a single gene within phenylpropanoid pathway results in significant alterations, at the root level, in theresponse towards mutualistic and pathogenic associates. These changes may constrain plant fitness and biomass production,which are of economic importance for perennial industrial crops such as poplar.
info:eu-repo/semantics/published
Databáze: OpenAIRE
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