Ingestion of lean meat elevates muscle inositol hexakisphosphate kinase 1 protein content independent of a distinct post-prandial circulating proteome in young adults with obesity
| Autor: | Brian S. Imai, Scott A. Paluska, Neale A. Tillin, Peter Watt, Richard W.A. Mackenzie, Martha Villegas-Montes, Joseph W. Beals, Nicholas A. Burd, Richie D. Barclay, Peter M. Yau, Alexander V. Ulanov, Michael De Lisio, Jenny Drnevich |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Meat Proteome Endocrinology Diabetes and Metabolism medicine.medical_treatment Muscle Proteins 030209 endocrinology & metabolism Article Body Mass Index 03 medical and health sciences Insulin-like growth factor Eating Young Adult 0302 clinical medicine Endocrinology Insulin resistance Thinness Internal medicine Ingestion Medicine Humans Obesity Muscle Skeletal Protein kinase B Phosphotransferases (Phosphate Group Acceptor) Inositol Hexakisphosphate Kinase 1 business.industry Insulin Age Factors Skeletal muscle Blood Proteins Middle Aged medicine.disease Postprandial Period Dietary Fats 030104 developmental biology medicine.anatomical_structure Glucose Homeostatic model assessment Female Insulin Resistance business Energy Metabolism |
| Zdroj: | Metabolism |
| Popis: | BACKGROUND: We have recently shown that a novel signalling kinase, inositol hexakisphosphate kinase 1 (IP6K1), is implicated in whole-body insulin resistance via its inhibitory action on Akt. Insulin and insulin like growth factor 1 (IGF-1) share many intracellular processes with both known to play a key role in glucose and protein metabolism in skeletal muscle. AIMS: We aimed to compare IGF/IP6K1/Akt signalling and the plasma proteomic signature in individuals with a range of BMIs after ingestion of lean meat. METHODS: Ten lean [Body mass index (BMI) (in kg/m(2)): 22.7 ± 0.4; Homeostatic model assessment of insulin resistance (HOMA(IR)): 1.36 ± 0.17], 10 overweight (BMI: 27.1 ± 0.5; HOMA(IR): 1.25 ± 0.11), and 10 obese (BMI: 35.9 ± 1.3; HOMA(IR): 5.82 ± 0.81) adults received primed continuous L-[ring-(13)C(6)]phenylalanine infusions. Blood and muscle biopsy samples were collected at 0 min (post-absorptive), 120 min and 300 min relative to the ingestion of 170 g pork loin (36 g protein and 5 g fat) to examine skeletal muscle protein signalling, plasma proteomic signatures, and whole-body phenylalanine disappearance rates (R(d)). RESULTS: Phenylalanine R(d) was not different in obese compared to lean individuals at all time points and was not responsive to a pork ingestion (basal, P = 0.056; 120 & 300min, P > 0.05). IP6K1 was elevated in obese individuals at 120 min post-prandial vs basal (P < 0.05). There were no acute differences plasma proteomic profiles between groups in the post-prandial state (P > 0.05). CONCLUSIONS: These data demonstrate, for the first time that muscle IP6K1 protein content is elevated after lean meat ingestion in obese adults, suggesting that IP6K1 may be contributing to the dysregulation of nutrient uptake in skeletal muscle. In addition, proteomic analysis showed no differences in proteomic signatures between obese, overweight or lean individuals. |
| Databáze: | OpenAIRE |
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