Fiber Reinforced Cartilage ECM Functionalized Bioinks for Functional Cartilage Tissue Engineering
| Autor: | Swetha Rathan, Léa Dejob, Daniel J. Kelly, Rossana Schipani, Benjamin Haffner, Matthias E. Möbius |
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| Rok vydání: | 2019 |
| Předmět: |
Cartilage
Articular Compressive Strength Alginates Swine Polyesters Biomedical Engineering Pharmaceutical Science Core Binding Factor Alpha 1 Subunit 02 engineering and technology 010402 general chemistry 01 natural sciences law.invention Biomaterials Extracellular matrix Transforming Growth Factor beta3 Tissue engineering law medicine Cartilaginous Tissue Animals Humans 3D bioprinting Tissue Engineering Chemistry Cartilage Regeneration (biology) Bioprinting Cell Differentiation Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Chondrogenesis Extracellular Matrix 0104 chemical sciences medicine.anatomical_structure Printing Three-Dimensional Ink Rheology 0210 nano-technology Collagen Type X Biofabrication Biomedical engineering |
| Zdroj: | Advanced Healthcare Materials |
| ISSN: | 2192-2659 2192-2640 |
| Popis: | Focal articular cartilage (AC) defects, if left untreated, can lead to debilitating diseases such as osteoarthritis. While several tissue engineering strategies have been developed to promote cartilage regeneration, it is still challenging to generate functional AC capable of sustaining high load-bearing environments. Here, a new class of cartilage extracellular matrix (cECM)-functionalized alginate bioink is developed for the bioprinting of cartilaginous tissues. The bioinks are 3D-printable, support mesenchymal stem cell (MSC) viability postprinting and robust chondrogenesis in vitro, with the highest levels of COLLII and ACAN expression observed in bioinks containing the highest concentration of cECM. Enhanced chondrogenesis in cECM-functionalized bioinks is also associated with progression along an endochondral-like pathway, as evident by increases in RUNX2 expression and calcium deposition in vitro. The bioinks loaded with MSCs and TGF-β3 are also found capable of supporting robust chondrogenesis, opening the possibility of using such bioinks for direct "print-and-implant" cartilage repair strategies. Finally, it is demonstrated that networks of 3D-printed polycaprolactone fibers with compressive modulus comparable to native AC can be used to mechanically reinforce these bioinks, with no loss in cell viability. It is envisioned that combinations of such biomaterials can be used in multiple-tool biofabrication strategies for the bioprinting of biomimetic cartilaginous implants. |
| Databáze: | OpenAIRE |
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