Investigation of a phage resistant Serratia entomophila strain (BC4B), establishment of generalised transduction and construction of S. entomophila RecA mutants
| Autor: | H. K. Mahanty, S Grkovic |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Serratia
Genotype DNA repair Ultraviolet Rays Restriction Mapping Biology medicine.disease_cause Microbiology Transduction Genetic Genetics medicine Escherichia coli Bacteriophages Cloning Molecular Molecular Biology Prophage Recombination Genetic Serratia entomophila Drug Resistance Microbial Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition Methyl Methanesulfonate Rec A Recombinases Subcloning Phenotype Genes Bacterial Mutagenesis Cosmid DNA Transposable Elements bacteria Transposon mutagenesis Virus Activation Homologous recombination Mutagens |
| Zdroj: | Moleculargeneral genetics : MGG. 250(3) |
| ISSN: | 0026-8925 |
| Popis: | A recA clone was isolated from a cosmid library of Serratia entomophila constructed in the Escherichia coli strain HB101. Subcloning and transposon mutagenesis were used to identify a 1.36 kb fragment containing the recA gene. A cloned recA mutation, generated by transposon mutagenesis and the replacement of a portion of the recA gene with an antibiotic resistance cassette, was introduced into the chromosome via a marker exchange technique. The recA strains created were deficient in DNA repair, homologous recombination and both the spontaneous and UV induction of prophages. S. entomophila recA strains showed continued pathogenicity towards the New Zealand grass grub, Costelytra zealandica. Simple procedures for further construction of S. entomophila recA strains have been demonstrated. |
| Databáze: | OpenAIRE |
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