Importance of Zinc Transporter 8 Autoantibody in the Diagnosis of Type 1 Diabetes in Latin Americans
| Autor: | Cintia Semzezem, Karla Fabiana Brasil Gomes, Rosa Tsuneshiro Fukui, Aritania S. Santos, Rodolfo Batista, Marcia Regina Soares Correia, Maria Elizabeth Rossi da Silva, Maria Rita Passos-Bueno |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_specialty Adolescent Genotype Science 030209 endocrinology & metabolism Zinc Transporter 8 Polymorphism Single Nucleotide Article White People Cohort Studies Young Adult 03 medical and health sciences 0302 clinical medicine Diabetes mellitus Internal medicine medicine Humans Genetic Predisposition to Disease Receptor-Like Protein Tyrosine Phosphatases Class 8 GENÉTICA DE POPULAÇÕES Genetic Association Studies Autoantibodies Type 1 diabetes Multidisciplinary biology SLC30A8 Glutamate Decarboxylase business.industry Autoantibody Radioimmunoassay medicine.disease Titer Diabetes Mellitus Type 1 030104 developmental biology Endocrinology biology.protein Medicine Female Antibody business Brazil |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-7 (2017) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | There is a scarcity of data of zinc transporter-8 autoantibody (ZnT8A) on mixed populations such as Brazilian. Therefore, we evaluated the relevance of ZnT8A for type 1 diabetes (T1D) diagnosis and the role of ZnT8 coding gene (SLC30A8) in T1D predisposition. Patients with T1D (n = 629; diabetes duration = 11 (6–16) years) and 651 controls were genotyped for SLC30A8 rs16889462 and rs2466295 variants (BeadXpress platform). ZnT8 triple antibody was measured by ELISA; glutamic acid decarboxylase (GAD65A) and protein tyrosine phosphatase (IA-2A) autoantibodies by radioimmunoassay. Results: Znt8A was detected in 68.7% of recent-onset T1D patients and 48.9% of the entire patient cohort, similar to GAD65A (68.3% and 47.2%) and IA-2A (64.8% and 42.4%) positivities respectively. ZnT8A was the only antibody in 8.4% of patients. Znt8A and IA2A frequencies and titers were independent of gender and ethnicity, whereas GAD65A titers were greater in females. The diabetes duration-dependent decline in ZnT8A frequency was similar to GAD65A and IA-2A. The SLC30A8 rs2466293 AG + GG genotypes were associated with T1D risk in non-European descents (56.2% × 42.9%; p = 0.018), and the GG genotype with higher ZnT8A titers in recent-onset T1D: 834.5 IU/mL (711.3–2190.0) × 281 IU/mL (10.7–726.8); p = 0.027. Conclusion ZnT8A detection increases T1D diagnosis rate even in mixed populations. SLC30A8 rs2466293 was associated with T1D predisposition in non-European descents. |
| Databáze: | OpenAIRE |
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