Lack of acidification in Mycobacterium phagosomes produced by exclusion of the vesicular proton-ATPase
| Autor: | John E. Heuser, Prasanta Chakraborty, David G. Russell, Helen L. Collins, Richard D. Allen, Paul H. Schlesinger, Pryce L. Haddix, Agnes K. Fok, Sheila Sturgill-Koszycki, Stephen L. Gluck |
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| Rok vydání: | 1994 |
| Předmět: |
Phagosome acidification
Immunoelectron microscopy Leishmania mexicana Vacuole Membrane Fusion Microbiology Mice Antigens CD Phagosomes Phagosome maturation Animals Microscopy Immunoelectron Phagosome Phagosome-lysosome fusion Multidisciplinary Membrane Glycoproteins biology Macrophages Lysosome-Associated Membrane Glycoproteins Mycobacterium tuberculosis Hydrogen-Ion Concentration biology.organism_classification Proton-Translocating ATPases Vacuoles Intracellular Mycobacterium Mycobacterium avium |
| Zdroj: | Science (New York, N.Y.). 263(5147) |
| ISSN: | 0036-8075 |
| Popis: | The success of Mycobacterium species as pathogens depends on their ability to maintain an infection inside the phagocytic vacuole of the macrophage. Although the bacteria are reported to modulate maturation of their intracellular vacuoles, the nature of such modifications is unknown. In this study, vacuoles formed around Mycobacterium avium failed to acidify below pH 6.3 to 6.5. Immunoelectron microscopy of infected macrophages and immunoblotting of isolated phagosomes showed that Mycobacterium vacuoles acquire the lysosomal membrane protein LAMP-1, but not the vesicular proton-adenosine triphosphatase (ATPase) responsible for phagosomal acidification. This suggests either a selective inhibition of fusion with proton-ATPase-containing vesicles or a rapid removal of the complex from Mycobacterium phagosomes. |
| Databáze: | OpenAIRE |
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