Time course of actin cytoskeleton stiffness and matrix adhesion molecules in human bronchial epithelial cell cultures
| Autor: | Blandine Doornaert, Stéphane Galiacy, Valérie Laurent, Emmanuelle Planus, Daniel Isabey, Gabriel Gras, Valérie Leblond, Chantal Lafuma |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Time Factors
biology Cell adhesion molecule Integrin Arp2/3 complex Actin remodeling Bronchi Epithelial Cells macromolecular substances Cell Biology Actin cytoskeleton Cadherins Actins Cell biology Cell Line Extracellular Matrix Extracellular matrix Actin remodeling of neurons biology.protein Humans Cytoskeleton Cell Adhesion Molecules Cell Division |
| Zdroj: | Experimental cell research. 287(2) |
| ISSN: | 0014-4827 |
| Popis: | Human bronchial epithelial (HBE) cells adhere to underlying extracellular matrix (ECM) via integrin-type transmembrane receptors. Integrins link the ECM to the cytoskeleton (CSK), establishing a mechanical continuum by which forces are transmitted between the outside and the inside of the cells. The present study investigates the time course of global and actin CSK stiffness of HBE cells (16HBE14o-) growing on various matrix substrates as a function of culture time until confluence, and the concomitant time course of F-actin and adhesion molecule distribution. Our results showed a progressive increase in actin CSK stiffness from cell seeding to confluence, related to acquisition of highly polymerized cortical and cytosolic F-actin organization and up-regulation of certain matrix ligands, such as beta 1-, alpha 5-, and alpha v-integrin subunit expression. Moreover, compared to fibrillar type I collagen, reticular type IV collagen used as matrix substrate, appeared to amplify actin CSK stiffness of HBE confluent cells probably in relation to up-regulation of alpha 3-integrin subunit. Taken together, these results support the concept of a close interaction among actin CSK stiffness, structural actin organization, specific integrin molecule involvement, cell spreading, and extracellular matrix. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect