Detection of Epstein-Barr Virus DNA in Gastric Biopsies of Pediatric Patients with Dyspepsia
| Autor: | Carolina Romo-González, Margarita Camorlinga-Ponce, Ezequiel M. Fuentes-Pananá, Abigail Morales-Sánchez, Lourdes Flores-Luna, Javier Torres, María G. Cárdenas-Mondragón |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
lcsh:Medicine Inflammation medicine.disease_cause Virus Article Serology hemic and lymphatic diseases medicine Gastric mucosa Immunology and Allergy gastric inflammation pediatric dyspepsia Molecular Biology General Immunology and Microbiology biology business.industry Stomach Epstein-Barr Virus lcsh:R Helicobacter pylori biology.organism_classification Epstein–Barr virus Infectious Diseases medicine.anatomical_structure Immunology helicobacter pylori nonatrophic gastritis Gastritis medicine.symptom business |
| Zdroj: | Pathogens Pathogens, Vol 9, Iss 623, p 623 (2020) Volume 9 Issue 8 |
| ISSN: | 2076-0817 |
| Popis: | In this study, we assessed the presence of Epstein-Barr virus (EBV) in gastric samples derived from pediatric patients with dyspeptic symptoms, aiming to understand whether EBV participates in the development of early gastric lesions influencing chronic inflammation, in conjunction with the Helicobacter pylori (Hp) bacterium. We analyzed EBV load in 236 gastric biopsies derived from 186 pediatric patients with chronic dyspepsia and compared it with EBV serology, Hp load and serology, and with immune cell infiltration. We found that 7.5% of patients were positive for EBV load, ranging from 240 to 29,685 genomic copies/&mu g of DNA. Hp genomic sequences were found in 24.7% of patients. EBV positive samples did not correlate with Hp status and were characterized by absent to moderate immune cell infiltration. To our knowledge, this is the first study addressing EBV load in the stomach in a large cohort of pediatric patients with dyspeptic symptoms, providing evidence of EBV localization in the gastric mucosa in early inflammatory lesions. The lack of correlation between EBV and both Hp infection and inflammation is perhaps explained by independent pathogenic mechanisms or because of the randomness of the gastritis sampling. This is also supported by a moderate association between EBV load and serology. |
| Databáze: | OpenAIRE |
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