Direct immobilization of cupredoxin azurin modified by site-directed mutagenesis on gold surface
Autor: | Jeong-Woo Choi, Sang-Uk Kim, Junhong Min, Byung-Keun Oh, Cheol-Heon Yea, Ravindra Pratap Singh, Young Jun Kim, Shin-Geon Choi |
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Rok vydání: | 2008 |
Předmět: |
Bioelectronics
Materials science technology industry and agriculture Nanotechnology Surface Plasmon Resonance Combinatorial chemistry Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials law.invention Adsorption Azurin Microscopy Scanning Tunneling law Pseudomonas aeruginosa Electrochemistry Mutagenesis Site-Directed Gold Scanning tunneling microscope Cyclic voltammetry Surface plasmon resonance Site-directed mutagenesis Instrumentation Linker |
Zdroj: | Ultramicroscopy. 108:1390-1395 |
ISSN: | 0304-3991 |
Popis: | For making efficient bioelectronic device, we have developed novel immobilization method of cupredoxin azurin modified on gold (Au) surface. A recombinant protein with cysteine residue by using site-directed mutagenesis was designed and then directly immobilized on Au surface without any chemical linker. The immobilization of the functionalized protein is confirmed by surface plasmon resonance (SPR) and its surface morphology is analyzed by scanning tunneling microscopy (STM). The immobilization efficiency has been increased about 75.6%, as compared to that of wild-type azurin. The electrochemical property of the fabricated thin film was investigated by the cyclic voltammetry (CV). As a result, cysteine-modified azurin can be used for making high-quality protein film, and applied to the fabrication of nano-scale bioelectronics. |
Databáze: | OpenAIRE |
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