Effects of alcohol consumption on biomarkers of oxidative damage to DNA and lipids in ethanol-fed pigs
| Autor: | M. Laurentie, Stéphanie Lagadu, Didier Pottier, Virginie Prevost, F. Petitpas, B. Hébert, François Sichel, M. Beljean |
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| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Alcohol Drinking Swine Oxidative phosphorylation Toxicology medicine.disease_cause Pathology and Forensic Medicine chemistry.chemical_compound Internal medicine Malondialdehyde medicine Animals Aspartate Aminotransferases Risk factor Chromatography High Pressure Liquid Liver injury Ethanol Cancer Deoxyguanosine Alanine Transaminase Cell Biology General Medicine medicine.disease Oxidative Stress Endocrinology medicine.anatomical_structure C-Reactive Protein chemistry Biochemistry 8-Hydroxy-2'-Deoxyguanosine Female Lipid Peroxidation Oxidative stress Biomarkers Respiratory tract DNA Damage |
| Zdroj: | Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie. 65(3) |
| ISSN: | 1618-1433 |
| Popis: | Chronic alcohol consumption is known to result in tissue injury, particularly in the liver, and is considered a major risk factor for cancers of the upper respiratory tract. Here we assessed the oxidative effects of subchronic ethanol consumption on DNA and lipids by measuring biomarkers 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) and malondialdehyde (MDA), respectively. Physiological responses of pigs (n = 4) administered ethanol in drinking water for 39 days were compared with those of water-fed pigs (n = 4). Alcoholisation resulted in serum ethanol concentration of 1.90 g L(-1) and in a moderate but significant increase in alanine aminotransferase activity, an index of liver injury. However, between the alcoholised and control groups there were no significant differences in the levels of 8-oxodG (8-oxodG per 10(6) 2'deoxyguanosine) from leucocytes (2.52 ± 0.42 Vs 2.39 ± 0.34) or from target organs, liver, cardia and oesophagus. Serum MDA levels were also similar in ethanol-fed pigs (0.33 ± 0.04 μM) and controls (0.28 ± 0.03 μM). Interestingly, levels of 8-oxodG in cardia were positively correlated with those in oesophagus (Spearman correlation coefficient R = 1, P0.0001). Our results suggest that alcohol consumption may not cause oxidative damage to DNA and lipids as measured by 8-oxodG and MDA, respectively. The duration of alcoholisation and the potential alcohol-induced nutritional deficiency may be critical determinants of ethanol toxicity. Relevant biomarkers, such as factors involved in sensitization to ethanol-induced oxidative stress are required to better elucidate the relationship between alcohol consumption, oxidative stress and carcinogenesis. |
| Databáze: | OpenAIRE |
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