A series of terpyridine containing flexible amino diethylacetate derivatives with large two-photon action cross-sections for effective mitochondrial imaging in living liver cancerous cells
| Autor: | Lei Hu, Xiaohe Tian, Mingzhu Zhang, Meng Zhao, Yingying Zhu, Qiru Xiong, Ran Jia |
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| Rok vydání: | 2017 |
| Předmět: |
Time Factors
Diethylamines Pyridines Mitochondria Liver 02 engineering and technology Mitochondrion 010402 general chemistry Crystallography X-Ray 01 natural sciences Fluorescence Analytical Chemistry chemistry.chemical_compound Imaging Three-Dimensional Two-photon excitation microscopy Cell Line Tumor Humans Instrumentation Spectroscopy Photons Staining and Labeling Liver Neoplasms Colocalization Chromophore 021001 nanoscience & nanotechnology Small molecule Atomic and Molecular Physics and Optics Endocytosis 0104 chemical sciences Biochemistry chemistry Biophysics Terpyridine 0210 nano-technology Biological imaging Intracellular |
| Zdroj: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 188 |
| ISSN: | 1873-3557 |
| Popis: | Small molecules possess large two-photon action cross sections (Φσ) are highly demanded for biological purpose. Herein, three novel terpyridine containing flexible amino diethylacetate organic small molecules (A1, A2 and A3) were rationally designed and their photophysical properties were investigated both experimentally and theoretically. The results revealed that the three chromophores possess large Φσ and remarkable Stokes' shift in high polar solvents, which are particularly benefit for further biological imaging application. One chromophore (A1) displayed an effective intracellular uptake against lung cancerous living cells A549. Colocalization studies suggested the internalized subcellular compartment was mitochondria. Consequently, chromophore A1 provides a promising platform to directly monitor mitochondria in living cells under two-photon confocal laser scanning microscopy. |
| Databáze: | OpenAIRE |
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