FLOWERING LOCUS T Protein May Act as the Long-Distance Florigenic Signal in the Cucurbits
| Autor: | Tony James Lough, Ming-Kuem Lin, Karla C Gendler, William J. Lucas, Ken Ichiro Taoka, Brett S. Phinney, Helene Belanger, Erika Varkonyi-Gasic, Young Jin Lee, Beatriz Xoconostle-Cázares, Eriko Miura, Richard A. Jorgensen |
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| Rok vydání: | 2007 |
| Předmět: |
Transcription
Genetic Photoperiod Genetic Vectors Meristem Molecular Sequence Data Arabidopsis Flowers Plant Science Chemical Fractionation Phloem Plant Viruses chemistry.chemical_compound Cucurbita Gene Expression Regulation Plant Plant virus Botany Amino Acid Sequence RNA Messenger Phylogeny Research Articles Plant Proteins photoperiodism Zucchini yellow mosaic virus Sequence Homology Amino Acid biology fungi food and beverages Cell Biology Plants Genetically Modified biology.organism_classification chemistry Cucurbita moschata Peptides Florigen Cucurbita maxima Signal Transduction |
| Zdroj: | The Plant Cell. 19:1488-1506 |
| ISSN: | 1532-298X |
| DOI: | 10.1105/tpc.107.051920 |
| Popis: | Cucurbita moschata, a cucurbit species responsive to inductive short-day (SD) photoperiods, and Zucchini yellow mosaic virus (ZYMV) were used to test whether long-distance movement of FLOWERING LOCUS T (FT) mRNA or FT is required for floral induction. Ectopic expression of FT by ZYMV was highly effective in mediating floral induction of long-day (LD)–treated plants. Moreover, the infection zone of ZYMV was far removed from floral meristems, suggesting that FT transcripts do not function as the florigenic signal in this system. Heterografting demonstrated efficient transmission of a florigenic signal from flowering Cucurbita maxima stocks to LD-grown C. moschata scions. Real-time RT-PCR performed on phloem sap collected from C. maxima stocks detected no FT transcripts, whereas mass spectrometry of phloem sap proteins revealed the presence of Cm-FTL1 and Cm-FTL2. Importantly, studies on LD- and SD-treated C. moschata plants established that Cmo-FTL1 and Cmo-FTL2 are regulated by photoperiod at the level of movement into the phloem and not by transcription. Finally, mass spectrometry of florally induced heterografted C. moschata scions revealed that C. maxima FT, but not FT mRNA, crossed the graft union in the phloem translocation stream. Collectively, these studies are consistent with FT functioning as a component of the florigenic signaling system in the cucurbits. |
| Databáze: | OpenAIRE |
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