microRNA-10b enhances pancreatic cancer cell invasion by suppressing TIP30 expression and promoting EGF and TGF-β actions
| Autor: | Ouyang, H, Gore, J, Deitz, S, Korc, M |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Cancer Research pancreatic cancer Gene Expression Mice Nude Antineoplastic Agents 03 medical and health sciences Erlotinib Hydrochloride Kruppel-Like Factor 4 Mice EGF receptor 0302 clinical medicine Acetyltransferases Transforming Growth Factor beta Cell Line Tumor Genetics Animals Humans Neoplasm Invasiveness Molecular Biology 030304 developmental biology 0303 health sciences Binding Sites Base Sequence Epidermal Growth Factor cell invasion Gene Expression Regulation Neoplastic Pancreatic Neoplasms MicroRNAs 030220 oncology & carcinogenesis Quinazolines Original Article RNA Interference Corrigendum miR-10b Neoplasm Transplantation Carcinoma Pancreatic Ductal Signal Transduction Transcription Factors |
| Zdroj: | Oncogene |
| ISSN: | 1476-5594 |
| Popis: | Increased microRNA-10b (miR-10b) expression in the cancer cells in pancreatic ductal adenocarcinoma (PDAC) is a marker of disease aggressiveness. In the present study, we determined that plasma miR-10b levels are significantly increased in PDAC patients by comparison with normal controls. By gene profiling, we identified potential targets downregulated by miR-10b, including Tat-interacting protein 30 (TIP30). Immunoblotting and luciferase reporter assays confirmed that TIP30 was a direct miR-10b target. Downregulation of TIP30 by miR-10b or siRNA-mediated silencing of TIP30 enhanced epidermal growth factor (EGF)-dependent invasion. The actions of miR-10b were abrogated by expressing a modified TIP30 cDNA resistant to miR-10b. EGF-induced EGF receptor (EGFR) tyrosine phosphorylation and extracellular signal–regulated kinase phosphorylation were enhanced by miR-10b, and these effects were mimicked by TIP30 silencing. The actions of EGF in the presence of miR-10b were blocked by EGFR kinase inhibition with erlotinib and by dual inhibition of PI3K (phosphatidylinositol 3′-kinase) and MEK. Moreover, miR-10b, EGF and transforming growth factor-beta (TGF-β) combined to markedly increase cell invasion, and this effect was blocked by the combination of erlotinib and SB505124, a type I TGF-β receptor inhibitor. miR-10b also enhanced the stimulatory effects of EGF and TGF-β on cell migration and epithelial–mesenchymal transition (EMT) and decreased the expression of RAP2A, EPHB2, KLF4 and NF1. Moreover, miR-10b overexpression accelerated pancreatic cancer cell (PCC) proliferation and tumor growth in an orthotopic model. Thus, plasma miR-10b levels may serve as a diagnostic marker in PDAC, whereas intra-tumoral miR-10b promotes PCC proliferation and invasion by suppressing TIP30, which enhances EGFR signaling, facilitates EGF–TGF-β cross-talk and enhances the expression of EMT-promoting genes, whereas decreasing the expression of several metastasis-suppressing genes. Therefore, therapeutic targeting of miR-10b in PDAC may interrupt growth-promoting deleterious EGF–TGF-β interactions and antagonize the metastatic process at various levels. |
| Databáze: | OpenAIRE |
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