Niemann-Pick C1 Heterogeneity of Bat Cells Controls Filovirus Tropism
Autor: | Andrea Marzi, Reiko Yoshida, Masahiro Kajihara, Hiroko Miyamoto, Alexander N. Freiberg, Manabu Igarashi, Yoshihiro Takadate, Hirohito Ogawa, Terence E. Hill, Wakako Furuyama, Tatsunari Kondoh, Heinz Feldmann, Ayato Takada, Masahiro Sato, Rashid Manzoor, Junki Maruyama |
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Rok vydání: | 2020 |
Předmět: |
Models
Molecular 0301 basic medicine viruses Host tropism Biology medicine.disease_cause Tropism General Biochemistry Genetics and Molecular Biology Marburg virus 03 medical and health sciences 0302 clinical medicine Niemann-Pick C1 Protein Chiroptera medicine Animals Humans Amino Acid Sequence Receptor lcsh:QH301-705.5 chemistry.chemical_classification Ebola virus Filoviridae Virology 030104 developmental biology lcsh:Biology (General) chemistry Cell culture NPC1 Glycoprotein 030217 neurology & neurosurgery |
Zdroj: | Cell Reports, Vol 30, Iss 2, Pp 308-319.e5 (2020) |
ISSN: | 2211-1247 |
Popis: | Summary: Fruit bats are suspected to be natural hosts of filoviruses, including Ebola virus (EBOV) and Marburg virus (MARV). Interestingly, however, previous studies suggest that these viruses have different tropisms depending on the bat species. Here, we show a molecular basis underlying the host-range restriction of filoviruses. We find that bat-derived cell lines FBKT1 and ZFBK13-76E show preferential susceptibility to EBOV and MARV, respectively, whereas the other bat cell lines tested are similarly infected with both viruses. In FBKT1 and ZFBK13-76E, unique amino acid (aa) sequences are found in the Niemann-Pick C1 (NPC1) protein, one of the cellular receptors interacting with the filovirus glycoprotein (GP). These aa residues, as well as a few aa differences between EBOV and MARV GPs, are crucial for the differential susceptibility to filoviruses. Taken together, our findings indicate that the heterogeneity of bat NPC1 orthologs is an important factor controlling filovirus species-specific host tropism. : Differential susceptibilities of bats to filoviruses have been suggested. Takadate et al. compare structures of the filovirus receptor among a variety of bat cell lines and discover a molecular mechanism determining their susceptibility to Ebola and Marburg viruses, providing information for understanding the ecology of filoviruses. Keywords: Ebola virus, Marburg virus, filovirus, bat, natural host, host range, glycoprotein, receptor, Niemann-Pick C1, virus-host interaction |
Databáze: | OpenAIRE |
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