Staining of HLA-DR, Iba1 and CD68 in human microglia reveals partially overlapping expression depending on cellular morphology and pathology
| Autor: | Karianne G. Schuurman, Corbert G. van Eden, Inge Huitinga, Jörg Hamann, Debbie A.E. Hendrickx |
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| Přispěvatelé: | Other departments, AII - Inflammatory diseases, Experimental Immunology, AII - Amsterdam institute for Infection and Immunity, Netherlands Institute for Neuroscience (NIN) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Pathology medicine.medical_specialty Multiple Sclerosis Immunology Hippocampus Antigens Differentiation Myelomonocytic Gene Expression Biology 03 medical and health sciences 0302 clinical medicine Alzheimer Disease Antigens CD HLA-DR medicine Immunology and Allergy Humans Aged Regulation of gene expression Aged 80 and over Microglia Staining and Labeling CD68 Multiple sclerosis Calcium-Binding Proteins Microfilament Proteins Brain HLA-DR Antigens Middle Aged medicine.disease Phenotype DNA-Binding Proteins 030104 developmental biology medicine.anatomical_structure Neurology Immunohistochemistry Female Neurology (clinical) 030217 neurology & neurosurgery Biomarkers |
| Zdroj: | Journal of neuroimmunology, 309, 12-22. Elsevier Journal of Neuroimmunology, 309, 12-22. Elsevier B.V. |
| ISSN: | 0165-5728 |
| Popis: | HLA-DR, Iba1 and CD68 are widely used microglia markers in human tissue. However, due to differences in gene regulation, they may identify different activation stages of microglia. Here, we directly compared the expression of HLA-DR, Iba1 and CD68 in microglia with different phenotypes, ranging from ramified to amoeboid, to foamy phagocytizing macrophages, in adjacent sections immunocytochemically double stained for two of the markers. Material was used from patients diagnosed with multiple sclerosis (MS) and Alzheimer's disease (AD) patients and control subjects because together they contain all the microglia activation stages in an acute and a chronic inflammatory setting. We found a similar, yet not identical, overall expression pattern. All three markers were expressed by ramified/amoeboid microglia around chronic active MS lesions, but overlap between HLA-DR and Iba1 was limited. Foamy macrophages in the demyelinating rims of active MS lesions of MS expressed more HLA-DR and CD68 than Iba1. All markers were expressed by small microglia accumulations (nodules) in MS NAWM. Dense core AD plaques in the hippocampus were mostly associated with microglia expressing HLA-DR. Diffuse AD plaques were not specifically associated with microglia at all. These results indicate that microglia markers have different potential for neuropathological analysis, with HLA-DR and CD68 reflecting immune activation and response to tissue damage, and Iba1 providing a marker more suited for structural studies in the absence of pathology. |
| Databáze: | OpenAIRE |
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