Liquid chromatographic determination of the cyanobacterial toxin beta-n-methylamino-L-alanine in algae food supplements, freshwater fish, and bottled water
| Autor: | Dorothea F. K. Rawn, Ben P.-Y. Lau, Peter M. Scott, Barbara Niedzwiadek |
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| Rok vydání: | 2009 |
| Předmět: |
Cyanobacteria
Hydrolyzed protein Food Contamination Microbiology Sensitivity and Specificity Fluorescence Hydrolysis Aquatic plant Animals Humans Chromatography biology Cyanobacteria Toxins Extraction (chemistry) Fishes Amino Acids Diamino Water Bottled water biology.organism_classification Seafood Dietary Supplements Freshwater fish Food Science Food contaminant Chromatography Liquid |
| Zdroj: | Journal of food protection. 72(8) |
| ISSN: | 0362-028X |
| Popis: | Beta-N-Methylamino-L-alanine (BMAA) is a neurotoxin originally found in cycad seeds and now known to be produced by many species of freshwater and marine cyanobacteria. We developed a method for its determination in blue-green algae (BGA) food supplements, freshwater fish, and bottled water by using a strong cation-exchange, solid-phase extraction column for cleanup after 0.3 M trichloroacetic acid extraction of BGA supplements and fish. Bottled water was applied directly onto the solid-phase extraction column. For analysis of carbonated water, sonication and pH adjustment to 1.5 were needed. To determine protein-bound BMAA, the protein pellet left after extraction of the BGA supplement and fish was hydrolyzed by boiling with 6 M hydrochloric acid; BMAA was cleaned up on a C18 column and a strong cation-exchange, solid-phase extraction column. Determination of BMAA was by liquid chromatography of the fluorescent derivative formed with 9-fluorenylmethyl chloroformate. The method was validated by recovery experiments using spiking levels of 1.0 to 10 microg/g for BGA supplements, 0.5 to 5.0 microg/g for fish, and 0.002 microg/g for bottled water; mean recoveries were in the range of 67 to 89% for BGA supplements and fish, and 59 to 92% for bottled water. Recoveries of BMAA from spiked extracts of hydrolyzed protein from BGA supplements and fish ranged from 66 to 83%. The cleanup developed provides a useful method for surveying foods and supplements for BMAA and protein-bound BMAA. |
| Databáze: | OpenAIRE |
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