Benzo[a]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests
| Autor: | Vesna Perić-Mataruga, Aleksandra Filipović, Marija Mrdaković, Milena Vlahović, Larisa Ilijin, Siniša Đurašević, Anja Grčić |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
esterase
Dispar Population polycyclic aromatic hydrocarbons (pahs) Zoology Environmental pollution Esterase General Biochemistry Genetics and Molecular Biology Hsp70 Lepidoptera genitalia Carboxylesterase chemistry.chemical_compound brain tissue Lymantria dispar parasitic diseases education lcsh:QH301-705.5 education.field_of_study biology lymantria dispar l fungi Lymantria dispar L benzo[a]pyrene hsp70 15. Life on land biology.organism_classification Brain tissue Benzo[a]pyrene Benzo(a)pyrene chemistry lcsh:Biology (General) 13. Climate action Polycyclic aromatic hydrocarbons (PAHs) Pyrene environmental pollution General Agricultural and Biological Sciences |
| Zdroj: | Archives of Biological Sciences, Vol 71, Iss 4, Pp 735-745 (2019) Archives of Biological Sciences |
| ISSN: | 1821-4339 0354-4664 |
| Popis: | Paper description: Anthropogenic activities are responsible for increased concentrations of the pollutant benzo[ a ]pyrene in the environment. Identifying molecular indicators of stress induced by benzo[ a ]pyrene in a widespread insect species, Lymantria dispar has great importance in pollution biomonitoring. Two populations of Lymantria dispar larvae from different habitats were fed on benzo[ a ]pyrene. Carboxylesterases, acetylcholinesterase and heat shock protein 70 from larva brain tissue served as molecular parameters of sensitivity to benzo[ a ]pyrene presence. Carboxylesterases and Hsp70 were sensitive to low benzo[ a ]pyrene concentrations and population pre-exposure history, with a dependent response recorded. Different pollution biomarkers are suitable for different Lymantria dispar populations. Abstract: Plant vegetation accumulates polycyclic aromatic hydrocarbons (PAHs) among which benzo[ a ]pyrene (B[ a ]P) is recognized as being very toxic, including cancerogenic. Lymantria dispar L. larvae are sensitive to changes in the environment, providing potential signs of pollutant presence. We examined the chronic effects of two concentrations of B[ a ]P on the activity of carboxylesterase (CaE), acetylcholinesterase (AChE) and heat shock protein 70 (Hsp70) levels in the brain tissue of two populations of L. dispar larvae, originating from unpolluted and polluted habitats. We found that the relative growth rate was significantly lower in both populations and that only larvae from polluted forests were sensitive to low B[ a ]P concentrations, exhibiting a significant increase in brain tissue CaE activity and Hsp70 concentration. AChE activity showed no changes in response to B[ a ]P exposure in either population. Examined biochemical parameters indicate that their sensitivity to chronic treatment with B[ a ]P was highly dependent on the pre-exposure history of L. dispar larvae, suggesting that they could be promising biomarkers of B[ a ]P and PAH pollution in forest ecosystems. https://doi.org/10.2298/ABS190620056G Received: June 20, 2019; Revised: July 30, 2019; Accepted: August 28, 2019; Published online: September 6, 2019 How to cite this article: Grcic A, Ilijin L, Mrdakovic M, Vlahovic M, Filipovic A, Đurasevic S, Peric-Mataruga V. Benzo[ a ]pyrene-induced changes in carboxylesterase, acetylcholinesterase and heat shock protein 70 of Lymantria dispar (Lepidoptera: Lymantriidae) from unpolluted and polluted forests. Arch Biol Sci. 2019;71(4):735-45. |
| Databáze: | OpenAIRE |
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