Ex Vivo Generation and Characterization of Human Hyaline and Elastic Cartilaginous Microtissues for Tissue Engineering Applications
| Autor: | Ana B. Paes, Rik Verplancke, José Darío Sánchez-López, Jan Vanfleteren, Óscar Darío García-García, Víctor Carriel, Daniel Durand-Herrera, Jesús Chato-Astrain, Fernando Campos, David Sánchez-Porras |
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| Přispěvatelé: | [Sánchez-Porras,D, Durand-Herrera,D, Chato-Astrain,J, García-García,OD, Campos,F, Carriel,V] Department of Histology, Tissue Engineering Group, Faculty of Medicine, University of Granada, Granada, Spain. [Sánchez-Porras,D, Carriel,V] Instituto de Investigación Biosanitaria ibs. GRANADA, Granada, Spain. [Sánchez-Porras,D] Doctoral Program in Biomedicine, Doctoral School, University of Granada, Granada, Spain. [Paes,AB] Master Program in Tissue Engineering and Advanced Therapies, International School for Postgraduate Studies, University of Granada, Granada, Spain. [Verplancke,R, Vanfleteren,J] Centre for Microsystems Technology (CMST), imec and Ghent University, Ghent, Belgium. [Sánchez-López,JD] Division of Maxillofacial Surgery, University Hospital Complex of Granada, Granada, Spain., This study was supported by grants FIS PI17/0393 and PI20/0318 from the Spanish Ministry of Science and Innovation (Instituto de Salud Carlos III), grants PI-0257-2017 and PE-0395- 2019 from Consejería de Salud y Familias, Junta de Andalucía, España, grant P18-RT-5059 from Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía, España, grant A-CTS-498-UGR18 from the University of Granada and Junta de Andalucía, España. It was co-funded by FEDER-ERDF funds. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Technology and Engineering
Hyaline cartilage extracellular matrix Human hyaline chondrocytes Organoides Medicine (miscellaneous) Microtissues Anatomy::Musculoskeletal System::Cartilage::Hyaline Cartilage [Medical Subject Headings] Anatomy::Cells::Connective Tissue Cells::Chondrocytes [Medical Subject Headings] Chemicals and Drugs::Carbohydrates::Polysaccharides::Sepharose [Medical Subject Headings] human hyaline chondrocytes General Biochemistry Genetics and Molecular Biology Article Extracellular matrix Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] Tissue engineering Anatomy::Musculoskeletal System::Cartilage::Elastic Cartilage [Medical Subject Headings] In vivo human elastic chondrocytes Elastic cartilage Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Survival [Medical Subject Headings] Psychiatry and Psychology::Psychological Phenomena and Processes::Psychophysiology::Arousal::Attention [Medical Subject Headings] Medicine and Health Sciences Viability assay Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Vital Statistics::Morbidity::Prevalence [Medical Subject Headings] lcsh:QH301-705.5 Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Culture Techniques::Cell Engineering::Tissue Engineering [Medical Subject Headings] Hyaline organoids Cartílago hialino Anatomy::Cells::Cellular Structures::Extracellular Space::Extracellular Matrix [Medical Subject Headings] High prevalence microtissues Disciplines and Occupations::Natural Science Disciplines::Biological Science Disciplines::Biotechnology::Biomimetics [Medical Subject Headings] Chemistry Biology and Life Sciences Chondrogenesis Human elastic chondrocytes Organoids lcsh:Biology (General) tissue engineering Cartílago elástico Ingeniería de tejidos Matriz extracelular Ex vivo Biomedical engineering |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) Biomedicines Biomedicines, Vol 9, Iss 292, p 292 (2021) Volume 9 Issue 3 BIOMEDICINES |
| ISSN: | 2227-9059 |
| Popis: | This study was supported by grants FIS PI17/0393 and PI20/0318 from the Spanish Ministry of Science and Innovation (Instituto de Salud Carlos III); grants PI-0257-2017 and PE-0395- 2019 from Consejería de Salud y Familias, Junta de Andalucía, España; grant P18-RT-5059 from Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía, España; grant A-CTS-498-UGR18 from the University of Granada and Junta de Andalucía, España. It was co-funded by FEDER-ERDF funds. Authors are grateful to Fabiola Bermejo Casares for the technical histological assistance. Special thanks to Ariane Ruyffelaert for her critical review and proofreading service. This work forms part of the doctoral thesis conducted by David Sánchez Porras (Doctoral Program in Biomedicine, Doctoral School, University of Granada, Spain). Considering the high prevalence of cartilage-associated pathologies, low self-repair capacity and limitations of current repair techniques, tissue engineering (TE) strategies have emerged as a promising alternative in this field. Three-dimensional culture techniques have gained attention in recent years, showing their ability to provide the most biomimetic environment for the cells under culture conditions, enabling the cells to fabricate natural, 3D functional microtissues (MTs). In this sense, the aim of this study was to generate, characterize and compare scaffold-free human hyaline and elastic cartilage-derived MTs (HC-MTs and EC-MTs, respectively) under expansion (EM) and chondrogenic media (CM). MTs were generated by using agarose microchips and evaluated ex vivo for 28 days. The MTs generated were subjected to morphometric assessment and cell viability, metabolic activity and histological analyses. Results suggest that the use of CM improves the biomimicry of the MTs obtained in terms of morphology, viability and extracellular matrix (ECM) synthesis with respect to the use of EM. Moreover, the overall results indicate a faster and more sensitive response of the EC-derived cells to the use of CM as compared to HC chondrocytes. Finally, future preclinical in vivo studies are still needed to determine the potential clinical usefulness of these novel advanced therapy products. Instituto de Salud Carlos III Spanish Government FIS PI17/0393 PI20/0318 Junta de Andalucia PI-0257-2017 PE-03952019 Junta de Andalucia P18-RT-5059 University of Granada A-CTS-498-UGR18 Junta de Andalucia A-CTS-498-UGR18 FEDER-ERDF funds |
| Databáze: | OpenAIRE |
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