pH-Responsible fluorescent carbon nanoparticles for tumor selective theranostics via pH-turn on/off fluorescence and photothermal effect in vivo and in vitro
| Autor: | Jung Eun Lee, Zihnil Adha Islamy Mazrad, Insik In, Sung Young Park, Ji Hoon Jeong, Eun Bi Kang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Indoles
Biocompatibility Infrared Rays 02 engineering and technology 010402 general chemistry Benzoates 01 natural sciences Fluorescence Theranostic Nanomedicine Mice In vivo Cell Line Tumor Neoplasms Animals Humans General Materials Science Chemistry Photothermal effect Hyperthermia Induced Hydrogen-Ion Concentration Photothermal therapy 021001 nanoscience & nanotechnology Carbon In vitro 0104 chemical sciences Cancer cell Biophysics Nanoparticles 0210 nano-technology Intracellular |
| Zdroj: | Nanoscale. 10:2512-2523 |
| ISSN: | 2040-3372 2040-3364 |
| DOI: | 10.1039/c7nr07900a |
| Popis: | We developed nanoparticles comprising a photothermal dye (IR825)-loaded carbonized zwitterionic polymer [FNP-I] as "switch-on" pH-responsive fluorescence probes to sense intracellular cancer cells and for near-infrared (NIR) controllable photothermal therapy (PTT) in vivo and in vitro. The fluorescent "off" of FNP-I was activated after reaching the cancer cell environment, where the zwitterionic compartment of FNP lost its hydrophobicity to induce PTT-mediated heat release of IR825 under NIR irradiation in the tumor. Approximately 100% of the IR825 was released from the FNP core to generate high thermal conversion to completely kill the cancer cells. Furthermore, after intravenous treatment of FNP-I into MDAMB-231-cell bearing mice, pH-responsive photothermal therapy was observed, achieving marked ablation of tumor cells with release of IR825 under tumor environment conditions. In addition, fluorescent signals were clearly found at the tumor site after 3 h, decreasing at the 6 h time point. The in vitro and in vivo detection system demonstrated good cellular uptake and biocompatibility as a potential imaging-guided photothermal therapy nanotool for cancer treatment. Interestingly, the synergism of the biosensor and PTT in single FNP-I platform led to more effective cancer cell killing than either monotherapy, providing a new approach for cancer treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|