Abnormal clot microstructure formed in blood containing HIT-like antibodies
| Autor: | John W. Weisel, M. Rowan Brown, Lubica Rauova, Bethan R. Thomas, P. Rhodri Williams, Karl Hawkins, Nafiseh Badiei, Rebecca J. Hambly, Catherine A. Thornton |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty 030204 cardiovascular system & hematology Platelet Factor 4 Fibrin Flow cytometry 03 medical and health sciences 0302 clinical medicine medicine Humans Platelet activation Whole blood medicine.diagnostic_test biology Chemistry Heparin Thrombosis Hematology Microstructure medicine.disease Thrombocytopenia 030220 oncology & carcinogenesis biology.protein Platelet factor 4 medicine.drug |
| Zdroj: | Thrombosis research. 193 |
| ISSN: | 1879-2472 |
| Popis: | INTRODUCTION Thrombosis is a severe and frequent complication of heparin-induced thrombocytopenia (HIT). However, there is currently no knowledge of the effects of HIT-like antibodies on the resulting microstructure of the formed clot, despite such information being linked to thrombotic events. We evaluate the effect of the addition of pathogenic HIT-like antibodies to blood on the resulting microstructure of the formed clot. MATERIALS AND METHODS Pathogenic HIT-like antibodies (KKO) and control antibodies (RTO) were added to samples of whole blood containing Unfractionated Heparin and Platelet Factor 4. The formed clot microstructure was investigated by rheological measurements (fractal dimension; df) and scanning electron microscopy (SEM), and platelet activation was measured by flow cytometry. RESULTS AND CONCLUSIONS Our results revealed striking effects of KKO on clot microstructure. A significant difference in df was found between samples containing KKO (df = 1.80) versus RTO (df = 1.74; p |
| Databáze: | OpenAIRE |
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