Redox-Sensitive Mapping of a Mouse Tumor Model Using Sparse Projection Sampling of Electron Paramagnetic Resonance
| Autor: | Hironobu Yasui, Shingo Matsumoto, Osamu Inanami, Kota Kimura, Hiroshi Hirata, Hitomi Nakano, Nami Iguchi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Materials science Physiology Clinical Biochemistry Biochemistry Projection (linear algebra) law.invention 03 medical and health sciences Mice Nuclear magnetic resonance Imaging Three-Dimensional Mouse xenograft Sampling (signal processing) law Neoplasms Animals Humans Mouse tumor mouse xenograft model Electron paramagnetic resonance Molecular Biology compressed sensing General Environmental Science 030102 biochemistry & molecular biology Phantoms Imaging Electron Spin Resonance Spectroscopy Cell Biology redox-sensitive mapping Redox sensitive Nitroxyl radicals electron paramagnetic resonance 030104 developmental biology nitroxyl radical General Earth and Planetary Sciences Oxidation-Reduction |
| Zdroj: | Antioxidantsredox signaling. 36(1-3) |
| ISSN: | 1557-7716 |
| Popis: | Aims: This work aimed to establish an accelerated imaging system for redox-sensitive mapping in a mouse tumor model using electron paramagnetic resonance (EPR) and nitroxyl radicals. Results: Sparse sampling of EPR spectral projections was demonstrated for a solution phantom. The reconstructed three-dimensional (3D) images with filtered back-projection (FBP) and compressed sensing image reconstruction were quantitatively assessed for the solution phantom. Mouse xenograft models of a human-derived pancreatic ductal adenocarcinoma cell line, MIA PaCa-2, were also measured for redox-sensitive mapping with the sparse sampling technique. Innovation: A short-lifetime redox-sensitive nitroxyl radical (N-15-labeled perdeuterated Tempone) could be measured to map the decay rates of the EPR signals for the mouse xenograft models. Acceleration of 3D EPR image acquisition broadened the choices of nitroxyl radical probes with various redox sensitivities to biological environments. Conclusion: Sparse sampling of EPR spectral projections accelerated image acquisition in the 3D redox-sensitive mapping of mouse tumor-bearing legs fourfold compared with conventional image acquisition with FBP. |
| Databáze: | OpenAIRE |
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