Evaluation of endogenous reference genes in Bactrocera cucurbitae by qPCR under different conditions
| Autor: | Li-Ming Niu, Zhi Gong, Li Lei, Yue-Guan Fu, Yanan Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Life Cycles Genetic Screens Melon Gene Identification and Analysis Gene Expression Artificial Gene Amplification and Extension Genes Insect Biochemistry Polymerase Chain Reaction Heat Shock Response Larvae Reference genes Gene expression Cellular Stress Responses Regulation of gene expression Genetics Multidisciplinary Tephritidae food and beverages Reference Standards Nucleic acids Real-time polymerase chain reaction Ribosomal RNA Cell Processes Medicine Insect Proteins Research Article Cell biology Cellular structures and organelles Science Biology Research and Analysis Methods Real-Time Polymerase Chain Reaction 03 medical and health sciences Extraction techniques Heat shock protein Bactrocera Animals HSP90 Heat-Shock Proteins Non-coding RNA Molecular Biology Techniques Gene Molecular Biology Biology and life sciences Gene Amplification biology.organism_classification RNA extraction Actins 030104 developmental biology Gene Expression Regulation RNA Ribosomes Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 13, Iss 12, p e0202829 (2018) |
| ISSN: | 1932-6203 |
| Popis: | Bactrocera cucurbitae (melon flies) are prominent invasive pests in southern China. To screen for a stable reference gene in melon flies suitable for comparing tissue samples subjected to different conditions in four categories (temperature, insect stage, days of age and gender), the expression of 12 candidate reference genes under different treatment conditions was analyzed by real-time fluorescent quantitative PCR. The results obtained from a comprehensive analysis with geNorm, NormFinder, BestKeeper and RefFinder software showed that the most stable reference gene was RPL60, and the least stable reference gene was actin-5. We used a heat shock protein gene (HSP-90) to verify the results, and the conclusion was consistent. When the reference gene RPL60 was used as the reference gene, the relative expression of HSP-90 was essentially constant with the prolongation of treatment time. When actin-5 was used, HSP-90 expression changed markedly with treatment time. The results of this study can be used for further research on gene expression inBactrocera cucurbitae. |
| Databáze: | OpenAIRE |
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