Novel systems for the application of isolated tensile, compressive, and shearing stimulation of distraction callus tissue

Autor: Lutz Claes, Nicholaus Meyers, Julian Schülke, Anita Ignatius
Rok vydání: 2017
Předmět:
Physiology
medicine.medical_treatment
Osteogenesis
Distraction
lcsh:Medicine
Stimulation
02 engineering and technology
Ossification
Osteotomy
Stiffness
0302 clinical medicine
Distraction
Medicine and Health Sciences
lcsh:Science
Mammals
030222 orthopedics
Multidisciplinary
Eukaryota
Ruminants
Physical Sciences
Vertebrates
Distraction osteogenesis
Engineering and Technology
Bone Remodeling
Anatomy
Research Article
Materials science
Histology
0206 medical engineering
Materials Science
Material Properties
Surgical and Invasive Medical Procedures
Bone healing
03 medical and health sciences
Ultimate tensile strength
Tissue Repair
medicine
Functional electrical stimulation
Animals
Mechanical Properties
Bone regeneration
Sheep
Functional Electrical Stimulation
Mechanical Engineering
lcsh:R
Organisms
Biology and Life Sciences
020601 biomedical engineering
Amniotes
lcsh:Q
Stress
Mechanical
Physiological Processes
Software
Actuators
Biomedical engineering
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 12, p e0189432 (2017)
ISSN: 1932-6203
Popis: Background Distraction osteogenesis is a procedure widely used for the correction of large bone defects. However, a high complication rate persists, likely due to insufficient stability during maturation. Numerical fracture healing models predict bone regeneration under different mechanical conditions allowing fixation stiffness optimization. However, most models apply a linear elastic material law inappropriate for the transient stresses/strains present during limb lengthening or segment transport. They are also often validated using in vivo osteotomy models lacking precise mechanical regulation due to the unavoidable stimulation of secondary interfragmentary motion during ambulation under finitely stiff fixation. Therefore, in order to create a robust numerical model of distraction osteogenesis, it is necessary to both characterize the new tissue’s viscoelasticity during distraction and determine the influence of strictly isolated stimulation in each loading mode (tension, compression, and shear) to account for potential differences in mechanical and histological response. Aim Two electromechanical fixators with integrated load cells were designed to precisely perform and monitor in vivo lateral distraction and isolated stimulation in sheep tibiae using a mobile, hydroxyapatite-coated titanium plate. The novel surgical procedure circumvents osteotomy, eliminating the undesirable and unquantifiable mechanical stimulation during ambulation. Methods After a 10-day post-surgery latency period, two 0.275 mm distraction steps were performed daily for 10 days. The load cell collected data before, during, and after each distraction step and was terminated after no less than one minute from the time of distraction. A 7-day consolidation period separated the distraction phase and 18-day stimulation phase. Stimulation was carried out in isolated tension, compression, or shear while recording force/time data. Each stimulation session consisted of 120 cycles with a magnitude of either 0.1 mm or 0.6 mm in the tension and compression groups and 1.0 mm in the shear group. The animals were euthanized after a 3-day holding period following stimulation. Results Our initial results show that the tissue progressively stiffens and maintains an increasingly large residual traction. The force curves during compressive stimulation show a progressive drift from compression toward tension. We hypothesize that this behavior may be due to the preferential flow of fluid outward from the tissue and a greater resistance to reabsorption during the plate’s return to the starting position.
Databáze: OpenAIRE
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