Novel SNPs in heat shock protein 70 gene and their association with sperm quality traits of Boer goats and Boer crosses
| Autor: | M. Murugaiyah, Halimatun Yaakub, Awis Qurni Sazili, Jothi Malar Panandam, Saeid Nikbin |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
endocrine system media_common.quotation_subject Male reproduction Semen Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Andrology Semen quality fluids and secretions Endocrinology Post-thaw semen quality Food Animals Fresh semen quality Animals HSP70 Heat-Shock Proteins Allele Sperm motility Single-strand conformation polymorphism media_common Genetics urogenital system Goats General Medicine Single nucleotide polymorphisms Sperm Spermatozoa Semen Analysis Animal Science and Zoology Reproduction |
| Zdroj: | Animal reproduction science. 146(3-4) |
| ISSN: | 1873-2232 |
| Popis: | The semen quality of bucks affects the reproduction performance of the herd and is influenced by genetic and non-genetic factors. Heat shock protein 70 (HSP70) is considered as an important gene affecting semen quality traits. The objectives of this study are to find single nucleotide polymorphisms in HSP70 coding region and their association with semen quality traits on Boer and Boer cross bucks. DNA isolated from 53 goats (36 pure South African Boer and 17 Boer crosses) was subjected to PCR amplification of the exon 1 region of the caprine HSP70 gene. Single-strand conformation polymorphism (SSCP) was used to detect polymorphisms and the variant DNA fragments were sequenced. Two synonymous SNPs (74A>C (ss836187517) and 191C>G (ss836187518)) were detected. Qualities of fresh and post-thaw semen were evaluated for sperm concentration, semen volume, sperm motility and velocity traits, live sperm percentage, and abnormal sperm rate. The C allele of ss836187517 and G allele of ss836187518 were at higher frequencies in both the breeds. The C allele of ss836187517 appeared to be the favorable allele for semen concentration, progressive motility of fresh semen, and motility and sperm lateral head displacement of post-thaw semen. A negative overdominance was observed for ss836187517 alleles on velocity traits of post-thaw semen. The C allele of ss836187518 was favorable for sperm concentration and progressive motility. Results herein suggest that the SNPs in HSP70 may affect on semen quality in tropical regions and specially on the potential of semen for freezing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect