Elucidation of the Molecular-Mechanisms and In Vivo Evaluation of the Anti-inflammatory Effect of Alginate-Derived Seleno-polymannuronate
| Autor: | Yanwen Peng, Zhang Yiyao, Xu Xu, Weiyang Bao, Jun Lu, Qiuxian Lai, Nan Cai, Hong Xu, Decheng Bi, Tong Li, Qingguo Han, Qiong Liu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lipopolysaccharide Alginates Anti-Inflammatory Agents Drug Evaluation Preclinical Nitric Oxide Synthase Type II Dinoprostone Nitric oxide Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Sulfation Glucuronic Acid medicine Macrophage Animals Humans Prostaglandin E2 chemistry.chemical_classification Inflammation Reactive oxygen species Mice Inbred BALB C biology Hexuronic Acids Macrophages NF-kappa B General Chemistry Molecular biology Nitric oxide synthase 030104 developmental biology chemistry Cyclooxygenase 2 030220 oncology & carcinogenesis biology.protein lipids (amino acids peptides and proteins) Female Mitogen-Activated Protein Kinases General Agricultural and Biological Sciences medicine.drug |
| Zdroj: | Journal of agricultural and food chemistry. 66(9) |
| ISSN: | 1520-5118 |
| Popis: | Alginate-derived polymannuronate (PM) is a type of polysaccharide found in edible brown seaweeds. Seleno-polymannuronate (Se-PM) was prepared from PM via synthesis using sulfation- and selenation-replacement reactions. The anti-inflammatory activity of Se-PM and its corresponding molecular mechanisms were investigated. In lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells, Se-PM significantly attenuated the production of nitric oxide (NO), prostaglandin E2 (PGE2), and reactive oxygen species (ROS); the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2); and the secretion of proinflammatory cytokines. Moreover, Se-PM remarkably suppressed the LPS-induced activation of the nuclear-factor (NF)-κB and mitogen-activated-protein-kinase (MAPK) signaling pathways in RAW264.7 cells. Furthermore, Se-PM also decreased the production of proinflammatory mediators in LPS-triggered primary murine macrophages. Additionally, Se-PM inhibited the inflammatory response in the a... |
| Databáze: | OpenAIRE |
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