Synthesis and characterization of molecularly imprinted polymers with metallic zinc center for enrofloxacin recognition
| Autor: | M. J. Bernad Bernad, J. Gracia Mora, J.J. Recillas Mota, J.A. Mayoral-Murillo |
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| Přispěvatelé: | Universidad Nacional Autónoma de México, Consejo Nacional de Ciencia y Tecnología (México), Consejo Mexiquense de Ciencia y Tecnología |
| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Enrofloxacin Polymers and Plastics General Chemical Engineering Molecularly imprinted polymer General Chemistry Polymer Oxazoline Biochemistry Styrene Metal complex chemistry.chemical_compound Molecular recognition chemistry Polymer chemistry Materials Chemistry Environmental Chemistry NIP Molecule Selectivity Azabis(oxazolines) Molecular imprinting polymer |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1873-166X 1381-5148 |
| Popis: | A molecularly imprinted polymer with a metallic center (MIPc) was prepared and characterized. The template molecule for MIPc was a complex [Zn(Aza-f)(Enr)]NO3·H2O, Com, based on the ligands: enrofloxacin (Enr) and azabis(oxazoline) functionalized with styrene. Com was characterized before preparing MIPc. Moreover, the polymer prepared via non-covalent (MIPe) was prepared using Enr as the template molecule while no template molecules were used to prepare the non-imprinted polymer (NIP). MIPc was characterized using different spectroscopic techniques. In addition, the polymer molecular recognition capacity was studied by using rebinding kinetic studies. The kinetics shows that MIPc reach the equilibrium before the MIPe and NIP and the amount of Enr adsorbed is approximately 5 times higher that MIPe and NIP. Scatchard plots analysis for NIP, MIPe and MIPc shows Kd = 0.4768, 0.020, 0.0067 (mmol L-1) respectively. In comparison with the MIPe the binding affinity is nearly 100 times higher taking into account the high affinity sites. The selectivity of MIPc for Enr was higher than that for ofloxacin or flumequin, isotherms were fit to the Langmuir-Freundlich model, and the Enr showed a value of Ko = 21.38 mM-1, while the ofloxacin had a value of 2.2 × 10-8 mM-1 and the flumequin of 1.6 × 10-5 mM-1. © 2013 Elsevier Ltd. All rights reserved. Thanks are due to the Dirección General de Asuntos del Personal Académico (DGAPA-UNAM) for support via Project PAPIIT-IN112805. JRM thanks Consejo Nacional de Ciencia y Tecnología (CONACYT) for a PhD scholarchip (No. 173224), PASPA UNAM and Consejo Mexiquense de Ciencia y Tecnología (COMECYT) for the scholarship (No. 08BTD0007). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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