Cross-reactive antibodies against dust mite-derived enolase induce neutrophilic airway inflammation
| Autor: | Lvxin Guan, Kexin Chang, Jianli Lin, Chengshen Hu, Zhigang Liu, Nanshan Zhong, Qing Xiong, Desheng Chen, Stephen Kwok-Wing Tsui, Jing Li, Dang Li, Xiaoyu Liu, Ping-Chang Yang, Nana Huang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Neutrophils Enolase Cross Reactions Antibodies Pathogenesis 03 medical and health sciences 0302 clinical medicine Mite medicine Animals Humans Asthma House dust mite Inflammation biology business.industry Pyroglyphidae Airway inflammation Dust biology.organism_classification medicine.disease respiratory tract diseases Disease Models Animal 030104 developmental biology 030228 respiratory system Phosphopyruvate Hydratase Immunology biology.protein Antibody business Airway |
| Zdroj: | The European respiratory journal. 57(1) |
| ISSN: | 1399-3003 |
| Popis: | Background and aimsNeutrophilic inflammation is a hallmark of some specific asthma phenotypes; its aetiology is not yet fully understood. House dust mite (HDM) is the most common factor in the pathogenesis of airway inflammation. This study aims to elucidate the role of cross-antibodies against HDM-derived factors in the development of neutrophilic inflammation in the airway.MethodsBlood samples were collected from asthma patients with chronic neutrophilic asthma for analysis of HDM-specific cross-reactive antibodies. The role of an antibody against HDM-derived enolase (EnoAb) in the impairment of airway epithelial barrier function and induction of airway inflammation was assessed in a cell culture model and an animal model.ResultsHigh similarity (72%) of the enolase gene sequences was identified between HDM and human. Serum EnoAb was detected in patients with chronic neutrophilic asthma. The EnoAb bound to airway epithelial cells to form complexes with enolase, which activated complement, impaired airway epithelial barrier functions and induced neutrophilic inflammation in the airway tissues.ConclusionsHDM-derived enolase can induce specific cross-antibodies in humans, which induce neutrophilic inflammation in the airway. |
| Databáze: | OpenAIRE |
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