The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin
| Autor: | Shujun Cai, Martin Pilhofer, Désirée Böck, Lu Gan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
In situ Heterochromatin Nuclear Functions Articles Cell Biology Biology Models Biological Linker DNA Nucleosomes 3. Good health Chromatin Folding (chemistry) 03 medical and health sciences 030104 developmental biology Biophysics Humans Nucleosome Molecular Biology Linker HeLa Cells |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, 29 (20) |
| ISSN: | 1059-1524 1939-4586 |
| DOI: | 10.1091/mbc.e18-05-0331 |
| Popis: | The in situ three-dimensional organization of chromatin at the nucleosome and oligonucleosome levels is unknown. Here we use cryo-electron tomography to determine the in situ structures of HeLa nucleosomes, which have canonical core structures and asymmetric, flexible linker DNA. Subtomogram remapping suggests that sequential nucleosomes in heterochromatin follow irregular paths at the oligonucleosome level. This basic principle of higher-order repressive chromatin folding is compatible with the conformational variability of the two linker DNAs at the single-nucleosome level. ISSN:1939-4586 ISSN:1059-1524 |
| Databáze: | OpenAIRE |
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