Oxygen and mechanical ventilation impede the functional properties of resident lung mesenchymal stromal cells
| Autor: | Steven R. Seidner, Margarita M. Vasquez, Desiree Wilson, Rolando Macias, Shamimunisa B. Mustafa, Teresa L. Johnson-Pais, Sartaj K. Siddiqui, Alvaro Moreira, Jonathon A. L. Gelfond |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Angiogenesis medicine.medical_treatment Gene Expression Pathology and Laboratory Medicine Endoplasmic Reticulum Biochemistry chemistry.chemical_compound 0302 clinical medicine Cell Movement Medicine and Health Sciences Cell Cycle and Cell Division Lung Energy-Producing Organelles Hyperoxia Mammals Multidisciplinary Secretory Pathway Eukaryota Cell Differentiation Animal Models 3. Good health Mitochondria Vascular endothelial growth factor Chemistry Experimental Organism Systems Cell Processes Vertebrates Physical Sciences Breathing Leporids Medicine Female Rabbits medicine.symptom Stem cell Cellular Structures and Organelles Research Article Chemical Elements Science Biology Bioenergetics Research and Analysis Methods Andrology 03 medical and health sciences Signs and Symptoms Diagnostic Medicine 030225 pediatrics medicine Genetics Animals Gene Regulation Cell Proliferation Mechanical ventilation Fetus Mesenchymal stem cell Organisms Biology and Life Sciences Mesenchymal Stem Cells Cell Biology Respiration Artificial Oxygen 030104 developmental biology chemistry Amniotes Animal Studies |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 3, p e0229521 (2020) |
| ISSN: | 1932-6203 |
| Popis: | Resident/endogenous mesenchymal stromal cells function to promote the normal development, growth, and repair of tissues. Following premature birth, the effects of routine neonatal care (e.g. oxygen support and mechanical ventilation) on the biological properties of lung endogenous mesenchymal stromal cells is (L-MSCs) is poorly understood. New Zealand white preterm rabbits were randomized into the following groups: (i) sacrificed at birth (Fetal), (ii) spontaneously breathing with 50% O2 for 4 hours (SB), or (iii) mechanical ventilation with 50% O2 for 4h (MV). At time of necropsy, L-MSCs were isolated, characterized, and compared. L-MSCs isolated from the MV group had decreased differentiation capacity, ability to form stem cell colonies, and expressed less vascular endothelial growth factor mRNA. Compared to Fetal L-MSCs, 98 and 458 genes were differentially expressed in the L-MSCs derived from the SB and MV groups, respectively. Gene ontology analysis revealed these genes were involved in key regulatory processes including cell cycle, cell division, and angiogenesis. Furthermore, the L-MSCs from the SB and MV groups had smaller mitochondria, nuclear changes, and distended endoplasmic reticula. Short-term hyperoxia/mechanical ventilation after birth alters the biological properties of L-MSCs and stimulates genomic changes that may impact their reparative potential. |
| Databáze: | OpenAIRE |
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