Specific Regulation of Noncanonical p38α Activation by Hsp90-Cdc37 Chaperone Complex in Cardiomyocyte
| Autor: | Yibin Wang, Jun Zhang, Peipei Ping, Asuka Ota, Jiahuai Han |
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| Rok vydání: | 2010 |
| Předmět: |
Time Factors
Chaperonins Cell Survival Physiology Lactams Macrocyclic MAP Kinase Kinase 3 Apoptosis Cell Cycle Proteins Mice Transgenic Transfection Article Mitogen-Activated Protein Kinase 14 Mice Chlorocebus aethiops Benzoquinones Animals Humans Myocytes Cardiac HSP90 Heat-Shock Proteins Phosphorylation Adaptor Proteins Signal Transducing MAPK14 biology Autophosphorylation HSP90-CDC37 chaperone complex Recombinant Proteins Rats Cell biology Enzyme Activation Oxidative Stress CDC37 Multiprotein Complexes Mitogen-activated protein kinase COS Cells Mutation biology.protein Chaperone complex RNA Interference Signal transduction Cardiology and Cardiovascular Medicine HeLa Cells Molecular Chaperones Protein Binding Signal Transduction |
| Zdroj: | Circulation Research. 106:1404-1412 |
| ISSN: | 1524-4571 0009-7330 |
| DOI: | 10.1161/circresaha.109.213769 |
| Popis: | Rationale : p38 is an important stress activated protein kinase involved in gene regulation, proliferation, differentiation, and cell death regulation in heart. p38 kinase activity can be induced through canonical pathway via upstream kinases or by noncanonical autophosphorylation. The intracellular p38 kinase activity is tightly regulated and maintained at low level under basal condition. The underlying regulatory mechanism for canonical p38 kinase activation is well-studied, but the regulation of noncanonical p38 autophosphorylation remains poorly understood. Objective : We investigated the molecular basis for the regulation of noncanonical p38 autophosphorylation and its potential functional impact in cardiomyocytes. Methods and Results : Using both proteomic and biochemical tools, we established that heat shock protein (Hsp)90-Cdc37 chaperones are part of the p38α signaling complex in mammalian cells both in vitro and in vivo. The Hsp90-Cdc37 chaperone complex interacts with p38 via direct binding between p38 and Cdc37. Cdc37 expression is both sufficient and necessary to suppress noncanonical p38 activation via autophosphorylation at either basal state or under TAB1 (TAK1 binding protein-1) induction. In contrast, Cdc37 expression has no impact on p38 activation by canonical upstream kinase MKK3 or oxidative stress. Furthermore, Hsp90 inhibition results in p38 activation via autophosphorylation, and p38 activity contribute to apoptotic cell death induced by Hsp90 inhibition. Conclusion : Our study has revealed a so far uncharacterized function of Hsp90-Cdc37 as an endogenous regulator of noncanonical p38 activity. |
| Databáze: | OpenAIRE |
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