Morbid obesity–related changes in the expression of lipid receptors, transporters, and HSL in human sperm

Autor: Berniza Calderón, José Manuel González-Casbas, Lydia Huerta, José I. Botella-Carretero, Antonia Martín-Hidalgo, María E. Casado
Rok vydání: 2019
Předmět:
Adult
CD36 Antigens
Male
0301 basic medicine
medicine.medical_specialty
Semen
03 medical and health sciences
0302 clinical medicine
Capacitation
Gamete Biology
Internal medicine
Genetics
medicine
Humans
Protein Isoforms
Receptor
Infertility
Male
Genetics (clinical)
Receptors
Scavenger
030219 obstetrics & reproductive medicine
Spermatozoon
biology
urogenital system
Cell Membrane
Lysosome-Associated Membrane Glycoproteins
food and beverages
Obstetrics and Gynecology
General Medicine
Sterol Esterase
Spermatozoa
Sperm
Obesity
Morbid
Cholesterol
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Gene Expression Regulation
Receptors
LDL
Reproductive Medicine
Lipotoxicity
ABCA1
LDL receptor
biology.protein
lipids (amino acids
peptides
and proteins)
Sperm Capacitation
ATP Binding Cassette Transporter 1
Developmental Biology
Zdroj: Journal of Assisted Reproduction and Genetics. 36:777-786
ISSN: 1573-7330
1058-0468
DOI: 10.1007/s10815-019-01406-z
Popis: OBJECTIVE: To study the location and expression of receptors (SR-BI/CLA-1, SR-BII, and LDLr) and transporter (ABCA1) involved in uptake and efflux of cholesterol in human spermatozoa and assess whether obesity alters its location/expression and whether this could be related to infertility. DESIGN: Observational study. SETTING: None PATIENT(S): Ten controls and 20 obese patients. INTERVENTION(S): Anthropometric parameters. Serum and semen samples were collected. MAIN OUTCOME MEASURE(S): Spermatozoon concentration, immunolocalization, and protein expression in semen. RESULTS: Spermatozoon concentration and motility was decreased in morbidly obese patients. SR-BI/CLA-1, SR-BII, LDLr, and ABCA1 are located in the spermatozoon cell membrane and the localization does not change between obese patients and controls. Control spermatozoa showed high SR-BI expression, and less expression for the rest of the receptors analyzed, indicating that SR-BI/CLA-1 is relevant in human spermatozoon cholesterol uptake/efflux. On the contrary, spermatozoa of obese patients showed less SR-BI/CLA-1 expression than controls, and more intense positive staining for SR-BII, LDLr, and ABCA1. Finally, human sperm expresses the 130- and 82-kDa hormone-sensitive lipase (HSL) isoforms. The 130-kDa isoform is expressed in the control sperm, and the expression disappears in the obese patients. CONCLUSION(S): The presence of lipid receptors/transporters and HSL in human spermatozoa suggests their role in the process of maturation/capacitation. The changes in the expression of lipid receptors/transporters and the lack of the 130-kDa HSL isoform in obese patients prevent the hydrolysis of cholesterol esters internalized by these receptors, and favor their accumulation in the cytoplasm of the spermatozoa that could contribute to lipotoxicity and infertility. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10815-019-01406-z) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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