Affinity chromatography of a sequence-specific DNA binding protein using teflon-linked oligonucleotides
| Autor: | Sharon L. Cavalier, Craig H. Duncan |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Chromatography
Base Sequence DNA synthesis Oligonucleotide Binding protein Oligonucleotides Biophysics Sequence-Specific DNA Binding Protein Cell Biology Biochemistry Chromatography Affinity DNA-Binding Proteins chemistry.chemical_compound chemistry Affinity chromatography Complementary DNA A-DNA Polytetrafluoroethylene Molecular Biology DNA |
| Zdroj: | Analytical Biochemistry. 169:104-108 |
| ISSN: | 0003-2697 |
| Popis: | An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated on an automated DNA synthesizer, contains high levels of attached DNA, and has superior mechanical properties. It should be generally useful for affinity chromatography of DNA binding proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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