Effect of Central Injection of Neostigmine on the Bacterial Endotoxin Induced Suppression of GnRH/LH Secretion in Ewes during the Follicular Phase of the Estrous Cycle

Autor: Joanna Bochenek, Andrzej Przemysław Herman, Hanna Antushevich, Janina Skipor, Bartosz Pawlina, Karolina Wojtulewicz, Agata Krawczyńska, Dorota Tomaszewska-Zaremba, Anna Herman
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Lipopolysaccharides
0301 basic medicine
Hydrocortisone
alpha7 Nicotinic Acetylcholine Receptor
medicine.medical_treatment
Gonadotropin-Releasing Hormone
lcsh:Chemistry
Follicular phase
lcsh:QH301-705.5
Spectroscopy
Chemistry
Interleukin
04 agricultural and veterinary sciences
General Medicine
reproduction disorders
Computer Science Applications
Cytokine
Follicular Phase
Hypothalamus
luteinizing hormone
Tumor necrosis factor alpha
Luteinizing hormone
medicine.drug
medicine.medical_specialty
endocrine system
Estrous Cycle
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Internal medicine
medicine
Physical and Theoretical Chemistry
Molecular Biology
Estrous cycle
Organic Chemistry
0402 animal and dairy science
neostigmine
040201 dairy & animal science
cytokines
Neostigmine
Endotoxins
030104 developmental biology
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
inflammation
GnRH
Cholinesterase Inhibitors
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 18, p 4598 (2019)
International Journal of Molecular Sciences
Volume 20
Issue 18
ISSN: 1422-0067
Popis: Induced by a bacterial infection, an immune/inflammatory challenge is a potent negative regulator of the reproduction process in females. The reduction of the synthesis of pro-inflammatory cytokine is considered as an effective strategy in the treatment of inflammatory induced neuroendocrine disorders. Therefore, the effect of direct administration of acetylcholinesterase inhibitor&mdash
neostigmine&mdash
into the third ventricle of the brain on the gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) secretions under basal and immune stress conditions was evaluated in this study. In the study, 24 adult, 2-years-old Blackhead ewes during the follicular phase of their estrous cycle were used. Immune stress was induced by the intravenous injection of LPS Escherichia coli in a dose of 400 ng/kg. Animals received an intracerebroventricular injection of neostigmine (1 mg/animal) 0.5 h before LPS/saline treatment. It was shown that central administration of neostigmine might prevent the inflammatory-dependent decrease of GnRH/LH secretion in ewes and it had a stimulatory effect on LH release. This central action of neostigmine is connected with its inhibitory action on local pro-inflammatory cytokines, such as interleukin (IL)-1&beta
IL-6, and tumor necrosis factor (TNF)&alpha
synthesis in the hypothalamus, which indicates the importance of this mediator in the inhibition of GnRH secretion during acute inflammation.
Databáze: OpenAIRE
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