Cytoprotection against neutrophil-delivered oxidant attack by antibiotics
| Autor: | Gianluigi Pastorino, Luciano Ottonello, Franco Dallegri, Carlo Sacchetti, Patrizia Dapino |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.drug_class Neutrophils Antibiotics Ceftazidime Biochemistry Microbiology Cell Line Ampicillin medicine Humans Pharmacology Piperacillin biology Dose-Response Relationship Drug Cytotoxins Penicillin G Cytoprotection Hypochlorous Acid Penicillin Cytolysis Myeloperoxidase biology.protein Tetradecanoylphorbol Acetate Oxidation-Reduction medicine.drug |
| Zdroj: | Biochemical pharmacology. 42(12) |
| ISSN: | 0006-2952 |
| Popis: | In the present study we have investigated the effect of six antibiotics (penicillin G, ceftazidime, cephotaxime, cephoperazon, ampicillin and piperacillin) on the neutrophil cytolytic activity by using a system constituted of phorbol-12-myristate-13-acetate-triggered neutrophils and 51Cr-labelled lymphoblastoid Daudi target cells. The results demonstrate that five of these drugs (ceftazidime, cephotaxime, cephoperazon, ampicillin and piperacillin) are capable of inhibiting the neutrophil cytolytic activity by inactivating the hypochlorous acid (HOCl) generated extracellularly by the myeloperoxidase pathway and crucial to the target cell lysis. Penicillin G had no effect on neutrophil-mediated cytolysis. Thus, these data demonstrate that ceftazidime, cephotaxime, cephoperazon, ampicillin and piperacillin lower the neutrophil-mediated target cell damage by a HOCl-scavenging mechanism, suggesting a possible cytoprotective role for these drugs during infections. |
| Databáze: | OpenAIRE |
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