Morphologic Design of Silver-Bearing Sugar-Based Polymer Nanoparticles for Uroepithelial Cell Binding and Antimicrobial Delivery
| Autor: | Mahmoud Elsabahy, Yue Song, Mei Dong, David A. Hunstad, Teri N. Hreha, Fuwu Zhang, Richen Li, Lu Su, Christina A. Collins, Yen-Nan Lin, Rachel A. Letteri, Yidan Shen, Karen L. Wooley, Sarosh Khan |
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| Rok vydání: | 2021 |
| Předmět: |
Silver
Polymers media_common.quotation_subject Nanoparticle Bioengineering 02 engineering and technology medicine.disease_cause Article Flow cytometry Amphiphile medicine General Materials Science Cytotoxicity Internalization Escherichia coli media_common chemistry.chemical_classification medicine.diagnostic_test Chemistry Mechanical Engineering General Chemistry Polymer 021001 nanoscience & nanotechnology Condensed Matter Physics Antimicrobial Anti-Bacterial Agents Biophysics Nanoparticles Sugars 0210 nano-technology |
| Zdroj: | Nano Lett |
| ISSN: | 1530-6992 1530-6984 |
| DOI: | 10.1021/acs.nanolett.1c00776 |
| Popis: | Platelet-like and cylindrical nanostructures from sugar-based polymers are designed to mimic the aspect ratio of bacteria and achieve uroepithelial cell binding and internalization, thereby improving their potential for local treatment of recurrent urinary tract infections. Polymer nanostructures, derived from amphiphilic block polymers composed of zwitterionic poly(d-glucose carbonate) and semicrystalline poly(l-lactide) segments, were constructed with morphologies that could be tuned to enhance uroepithelial cell binding. These nanoparticles exhibited negligible cytotoxicity, immunotoxicity, and cytokine adsorption, while also offering substantial silver cation loading capacity, extended release, and in vitro antimicrobial activity (as effective as free silver cations) against uropathogenic Escherichia coli. In comparison to spherical analogues, cylindrical and platelet-like nanostructures engaged in significantly higher association with uroepithelial cells, as measured by flow cytometry; despite their larger size, platelet-like nanostructures maintained the capacity for cell internalization. This work establishes initial evidence of degradable platelet-shaped nanostructures as versatile therapeutic carriers for treatment of epithelial infections. |
| Databáze: | OpenAIRE |
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