Multiparameter immunohistochemistry analysis of HIV DNA, RNA and immune checkpoints in lymph node tissue
| Autor: | Mauricio González-Navarro, Jacob D. Estes, Claire Deleage, Paul U. Cameron, Rachel D. Pascoe, Candani Tutuka, Michael Gonzales, Fernanda Torres-Ruiz, Marzena Walkiewicz, Zuwena A. Richardson, Vanessa A. Evans, Samuel Roberts-Thomson, Perla M. Del Río-Estrada, Sharon R Lewin, Gustavo Reyes-Terán |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Immunology
HIV Infections In situ hybridization Biology T-Lymphocytes Regulatory Article chemistry.chemical_compound Jurkat Cells Immune system Predictive Value of Tests medicine Immunology and Allergy Humans Multiplex Lymph node Immune Checkpoint Inhibitors In Situ Hybridization RNA FOXP3 virus diseases HIV Virology Immunohistochemistry medicine.anatomical_structure chemistry DNA Viral Latent Infection RNA Viral Lymph Nodes DNA |
| Zdroj: | J Immunol Methods |
| Popis: | The main barrier to a cure for HIV is the persistence of long-lived and proliferating latently infected CD4+ T-cells despite antiretroviral therapy (ART). Latency is well characterized in multiple CD4+ T-cell subsets, however, the contribution of regulatory T-cells (Tregs) expressing FoxP3 as well as immune checkpoints (ICs) PD-1 and CTLA-4 as targets for productive and latent HIV infection in people living with HIV on suppressive ART is less well defined. We used multiplex detection of HIV DNA and RNA with immunohistochemistry (mIHC) on formalin-fixed paraffin embedded (FFPE) cells to simultaneously detect HIV RNA and DNA and cellular markers. HIV DNA and RNA were detected by in situ hybridization (ISH) (RNA/DNAscope) and IHC was used to detect cellular markers (CD4, PD-1, FoxP3, and CTLA-4) by incorporating the tyramide system amplification (TSA) system. We evaluated latently infected cell lines, a primary cell model of HIV latency and excisional lymph node (LN) biopsies collected from people living with HIV (PLWH) on and off ART. We clearly detected infected cells that coexpressed HIV RNA and DNA (active replication) and DNA only (latently infected cells) in combination with IHC markers in the in vitro infection model as well as LN tissue from PLWH both on and off ART. Combining ISH targeting HIV RNA and DNA with IHC provides a platform to detect and quantify HIV persistence within cells identified by multiple markers in tissue samples from PLWH on ART or to study HIV latency. |
| Databáze: | OpenAIRE |
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