Identification of proteins associated with Fusarium crown rot resistance in wheat using label-free quantification analysis
| Autor: | Yong-zhi Qi, Jun Ma, Jingjing Jin, Wenchao Zhen, Shuonan Duan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Fusarium
Agriculture (General) Fusarium crown rot Plant Science Biochemistry differential expression S1-972 Cell wall chemistry.chemical_compound multiple time points Food Animals Chitin wheat Glycosyltransferase Gene proteomic Ecology biology RuBisCO food and beverages biology.organism_classification Label-free quantification chemistry Chitinase biology.protein Animal Science and Zoology Agronomy and Crop Science Food Science |
| Zdroj: | Journal of Integrative Agriculture, Vol 20, Iss 12, Pp 3209-3221 (2021) |
| ISSN: | 2095-3119 |
| Popis: | Fusarium crown rot (FCR), typically caused by Fusarium pseudograminearum, is a severe soil-borne disease that, in recent years, has become an emerging threat to Chinese wheat crops. For the first time in this study, we investigated and compared the proteomic characteristics of two Chinese wheat varieties (04 Zhong 36 and Xinmai 26) at 24, 48, and 72 h post-inoculation using label-free quantitative proteomic analysis. A total of 9 234 proteins were successfully quantified, of which 783 were differentially expressed after inoculation. These proteins were mainly involved in metabolic, single-organism, and cellular processes. Thirty-three proteins associated with defense, cell wall formation, photosynthesis, etc., showed consistently different expression between the two genotypes at multiple time points. In particular, chitinase, which degrades chitin in the fungal cell wall and limits fungal growth, was exclusively and consistently upregulated in 04 Zhong 36 across the three time points. Other proteins such as flavonoid O-methyltransferase, glycosyltransferase, and peroxidase were only upregulated in 04 Zhong 36, and proteins, including the berberine bridge enzyme and rubisco large subunit-binding protein, were specifically downregulated in Xinmai 26. The expression of transcripts encoding eight selected proteins through qRT-PCR analysis supported the proteomic profiles. Overall, the results of this study allow us to understand FCR resistance in wheat at the protein level. Some proteins and their corresponding genes may be useful resources for the genetic improvement of FCR resistance in wheat. |
| Databáze: | OpenAIRE |
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