Mycobacterium smegmatis proteoliposome induce protection in a murine progressive pulmonary tuberculosis model
| Autor: | María E. Sarmiento, Mohd Nor Norazmi, Sonsire Fernández, Rogelio Hernández-Pando, Dulce Mata Espinoza, Frank Camacho, Jorge Alberto Barrios Payan, Fátima Reyes, Nadine Alvarez, Alina Puig, Reinier Borrero, Reynaldo Acevedo, Yanely Tirado, Armando Acosta, Ramlah Kadir, Alicia Aguilar, José Luis Monereo Pérez, María de los Angeles García |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Tuberculosis Proteolipids Mycobacterium smegmatis Immunology Disease Microbiology Mycobacterium tuberculosis 03 medical and health sciences Adjuvants Immunologic Antigen Pneumonia Bacterial medicine Animals Tuberculosis Vaccines Tuberculosis Pulmonary Colony-forming unit Mice Inbred BALB C Lung biology Transmission (medicine) business.industry biology.organism_classification medicine.disease Bacterial Load Disease Models Animal 030104 developmental biology Infectious Diseases medicine.anatomical_structure BCG Vaccine Disease Progression Alum Compounds business |
| Zdroj: | Tuberculosis. 101:44-48 |
| ISSN: | 1472-9792 |
| Popis: | Summary Tuberculosis (TB) remains an important cause of mortality and morbidity. The TB vaccine, BCG, is not fully protective against the adult form of the disease and is unable to prevent its transmission although it is still useful against severe childhood TB. Hence, the search for new vaccines is of great interest. In a previous study, we have shown that proteoliposomes obtained from Mycobacterium smegmatis (PLMs) induced cross reactive humoral and cellular response against Mycobacterium tuberculosis (Mtb) antigens. With the objective to evaluate the protective capability of PLMs, a murine model of progressive pulmonary TB was used. Animals immunized with PLMs with and without alum (PLMs/PLMsAL respectively) showed protection compared to non-immunized animals. Mice immunized with PLMsAL induced similar protection as that of BCG. Animals immunized with BCG, PLMs and PLMsAL showed a significant decrease in tissue damage (percentage of pneumonic area/lung) compared to non-immunized animals, with a more prominent effect in BCG vaccinated mice. The protective effect of the administration of PLMs in mice supports its future evaluation as experimental vaccine candidate against Mtb. |
| Databáze: | OpenAIRE |
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