Effect of Berberine on Barrier Function in a Human Retinal Pigment Epithelial Cell Line
| Autor: | Lian-Shun Zheng, Hu-Shan Cui, Yoriko Hayasaka, Seiji Hayasaka, Zai-Long Chi, Xue-Yun Zhang |
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| Rok vydání: | 2007 |
| Předmět: |
Cell Membrane Permeability
Berberine Interleukin-1beta Retinal pigment epithelial cell complex mixtures Cell Line chemistry.chemical_compound Blood-Retinal Barrier Botany Electric Impedance Humans Pigment Epithelium of Eye Horseradish Peroxidase Barrier function Dose-Response Relationship Drug Chemistry Alkaloid Biological Transport General Medicine Cell biology Electrophysiology Interleukin 1β Ophthalmology Medicinal herbs Fluorescein Line (text file) |
| Zdroj: | Japanese Journal of Ophthalmology. 51:64-67 |
| ISSN: | 1613-2246 0021-5155 |
| DOI: | 10.1007/s10384-006-0395-0 |
| Popis: | To examine the effects of berberine, an alkaloid isolated from some medicinal herbs, on the disruption of the barrier function in a human retinal pigment epithelial cell line (ARPE-19) stimulated with interleukin-1beta (IL-1beta).ARPE-19 cells were cultured to confluence. Berberine and IL-1beta were added to the medium. Barrier functions were evaluated by measuring transepithelial electrical resistance (TER) and the permeability to horseradish peroxidase (HRP) and sodium fluorescein (SF).Berberine dose-dependently inhibited decreased TER and increased the permeability to HRP and SF in the cells stimulated with IL-1beta.Berberine dose-dependently inhibited the disruption of the barrier function in the ARPE-19 cell line induced by IL-1beta. |
| Databáze: | OpenAIRE |
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