Resident pleural macrophages are key orchestrators of neutrophil recruitment in pleural inflammation
| Autor: | Richard A. Lang, David C. Kluth, Kris Houlberg, Dave Walbaum, Tiina Kipari, Jeremy Hughes, Deborah A. Sawatzky, Jean Francois Cailhier, Simon Watson, John Savill, Spike Clay |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Pulmonary and Respiratory Medicine
Pathology medicine.medical_specialty Neutrophile Inflammation Mice Transgenic Critical Care and Intensive Care Medicine Carrageenan Mice Intensive care medicine Animals Diphtheria Toxin Pleurisy Diphtheria toxin biology business.industry Macrophages Pleural cavity respiratory system medicine.disease respiratory tract diseases medicine.anatomical_structure Integrin alpha M Neutrophil Infiltration Immunology biology.protein Pleura medicine.symptom business Infiltration (medical) E. Pleural Disorders |
| Zdroj: | Cailhier, J F, Sawatzky, D A, Kipari, T, Houlberg, K, Walbaum, D, Watson, S, Lang, R A, Clay, S, Kluth, D, Savill, J & Hughes, J 2006, ' Resident pleural macrophages are key orchestrators of neutrophil recruitment in pleural inflammation ', American Journal of Respiratory and Critical Care Medicine, vol. 173, no. 5, pp. 540-7 . https://doi.org/10.1164/rccm.200504-538OC |
| DOI: | 10.1164/rccm.200504-538OC |
| Popis: | Rationale: The role played by resident pleural macrophages in the initiation of pleural inflammation is currently unclear.Objective: To evaluate the role of resident pleural macrophages in the initiation of inflammation.Methods: We have used a conditional macrophage ablation strategy to determine the role of resident pleural macrophages in the regulation of neutrophil recruitment in a murine model of experimental pleurisy induced by the administration of carrageenan and formalin- fixed Staphylococcus aureus.Measurements and Main Results: Conditional macrophage ablation mice express the human diphtheria toxin receptor under the control of the CD11b promoter such that the administration of diphtheria toxin induces ablation of nearly 97% of resident macrophages. Ablation of resident pleural macrophages before the administration of carrageenan or S. aureus dramatically reduced neutrophil influx into the pleural cavity. In the carrageenan model, the reduction in neutrophil infiltration was associated with marked early reduction in the level of macrophage inflammatory protein 2 as well as reduced levels of various cytokines, including tumor necrosis factor α, interleukin 6, and interleukin 10. Adoptive transfer of nontransgenic macrophages partially restored neutrophil infiltration. We also stimulated macrophage-depleted and nondepleted pleural cell populations with carrageenan in vitro and determined the production of chemokines and cytokines. Chemokine and cytokine production was markedly reduced by macrophage depletion, reinforcing the role of resident pleural macrophages in the generation of mediators that initiate acute inflammation.Conclusion: These studies indicate a critical role for resident pleural macrophages in sensing perturbation to the local microenvironment and orchestrating subsequent neutrophil infiltration.Read More: http://www.atsjournals.org/doi/abs/10.1164/rccm.200504-538OC |
| Databáze: | OpenAIRE |
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