Effect of breast cyst fluid on oestrogen 17-oxidoreductase activity in MCF-7 breast cancer cells
| Autor: | M.W. Ghilchik, Michael J. Reed, N. G. Coldham, S. Islam, L.C. Lai, V.H.T. James |
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| Rok vydání: | 1990 |
| Předmět: |
Cancer Research
medicine.medical_specialty Estrone medicine.drug_class medicine.medical_treatment Mammary gland Breast Neoplasms Stimulation Biology medicine.disease_cause Steroid Estradiol Dehydrogenases Internal medicine Mole Tumor Cells Cultured medicine Humans Fibrocystic Breast Disease skin and connective tissue diseases Chromatography High Pressure Liquid chemistry.chemical_classification Estradiol Estrogens Body Fluids Endocrinology medicine.anatomical_structure Enzyme Oncology MCF-7 chemistry Estrogen Chromatography Gel Female Carcinogenesis Dialysis Cell Division |
| Zdroj: | Cancer Letters. 55:165-169 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/0304-3835(90)90028-v |
| Popis: | Breast cyst fluid (BCF) was found to stimulate oestrogen 17-oxidoreductase activity in the reductive direction, i.e., conversion of oestrone (E1) to oestradiol (E2), in MCF-7 breast cancer cells. Dialysis of BCF revealed that this property of BCF was present in both dialysed BCF and dialysate, implying that both high and low mol. wt. substances were responsible for stimulating E1 to E2 conversion. Gel filtration of dialysed BCF revealed that the high mol. wt. substances responsible for the stimulation of E1 to E2 conversion had mol. wts. of approximately 11 kD and 68 kD. This property of BCF would serve to increase the concentration of E2, a steroid which may play a role in mammary carcinogenesis. |
| Databáze: | OpenAIRE |
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