Filoviruses Infect Rhesus Macaque Synoviocytes in Vivo and Primary Human Synoviocytes in Vitro
| Autor: | Oscar Rojas, Timothy K. Cooper, David X. Liu, Dawn M. Gerhardt, Randy Hart, Amanda M.W. Hischak, Lisa E. Hensley, Peter B. Jahrling, J. Kyle Bohannon, Katie R. Hagen, John G. Bernbaum, Reed F. Johnson, Donna L. Perry, James Logue, Richard S. Bennett, Ian Crozier |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases viruses Filoviridae In situ hybridization medicine.disease_cause Immunofluorescence Article Pathology and Forensic Medicine Marburg virus Pathogenesis 03 medical and health sciences 0302 clinical medicine Immune system Filoviridae Infections Medicine Animals Humans 030212 general & internal medicine Cells Cultured Ebola virus medicine.diagnostic_test biology business.industry virus diseases biology.organism_classification Virology Macaca mulatta Synoviocytes Rhesus macaque 030104 developmental biology business |
| Zdroj: | Am J Pathol |
| ISSN: | 1525-2191 |
| Popis: | The most commonly reported symptom of post–Ebola virus disease syndrome in survivors is arthralgia, yet involvement of the joints in acute or convalescent Ebola virus infection is not well characterized in human patients or animal models. Through immunohistochemistry, we found that the lining synovial intima of the stifle (knee) is a target for acute infection by Ebola virus/Kikwit, Ebola virus/Makona-C05, and Marburg virus/Angola in the rhesus macaque model. Furthermore, histologic analysis, immunohistochemistry, RNAscope in situ hybridization, and transmission electron microscopy showed that synoviocytes of the stifle, shoulder, and hip are a target for mouse-adapted Ebola virus/Yambuku-Mayinga infection during acute disease in rhesus macaques. A time course of infection study with Ebola virus/Kikwit found that the large joint synovium became immunopositive beginning on postinfection day 6. In total, the synovium of 28 of 30 rhesus macaques with terminal filovirus disease had evidence of infection (64 of 96 joints examined). On the basis of immunofluorescence, infected cell types included CD68(+) type A (macrophage-like) synoviocytes and CD44(+) type B (fibroblast-like) synoviocytes. Cultured primary human fibroblast–like synoviocytes were permissive to infection with Ebola and Marburg viruses in vitro. Because synovial joints include immune privileged sites, these findings are significant for future investigations of filovirus pathogenesis and persistence as well as arthralgias in acute and convalescent filovirus disease. |
| Databáze: | OpenAIRE |
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