Correlation between the Anti-Virus-Induced Cytopathic Effect Activity of Interferon-α Subtypes and Induction of MxA ProteinIn Vitro
| Autor: | Didier Hober, Pierre-Emmanuel Lobert, Jeanne Harvey, Vincent Chieux, Cécile Schanen |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Myxovirus Resistance Proteins
viruses Statistics as Topic Immunology Antiviral protein Microbiology Vesicular stomatitis Indiana virus Cell Line Cytopathogenic Effect Viral GTP-Binding Proteins Virology medicine Humans Cytopathic effect biology Amnion Interferon-alpha Biological activity biology.organism_classification Molecular biology In vitro medicine.anatomical_structure Cell culture Vesicular stomatitis virus Luminescent Measurements Intracellular |
| Zdroj: | Microbiology and Immunology. 50:19-24 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.2006.tb03766.x |
| Popis: | There are several interferon-alpha (IFN-alpha) subtypes. Mechanism of disparity in biological effects among members of IFN-alpha subtypes remains unexplained. Biological activity of IFN-alpha is mediated in part by induction of intracellular antiviral proteins. We studied whether differences in biologic effects of IFN-alpha subtypes may rely on their antiviral protein inducing effect. Intracellular induction of MxA protein and anti-virus-induced cytopathic effect (CPE) activity of 11 IFN-alpha subtypes in human amnion WISH cells have been studied. MxA protein quantitation in cell lysates was performed by immunochemiluminescence assay and anti-virus-induced CPE activity was assessed by protection against vesicular stomatitis virus (VSV)-induced CPE. Range of MxA values was high when cells were treated with 10 and 100 IU/ml of each IFN-alpha subtype. Levels of MxA correlated with anti-VSV-induced CPE obtained with 10 IU/ml IFN-alpha subtype. Together our data show a disparity in MxA-inducing activity of IFN-alpha subtypes and suggest that differences in anti-VSV-induced CPE of IFN-alpha subtypes in WISH cells can be related to their different ability to induce MxA. |
| Databáze: | OpenAIRE |
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