Effects of clinorotation on the polysaccharide content of resynthesized walls of protoplasts
| Autor: | E.M. Nedukha |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Atmospheric Science
Cytoplasmic calcium Rotation Aerospace Engineering Brassica Polysaccharide Cell wall chemistry.chemical_compound Cell Wall Polysaccharides Cellulose Glucans Weightlessness Simulation chemistry.chemical_classification Protoplasts fungi Callose food and beverages Astronomy and Astrophysics Protoplast Geophysics chemistry Simulated microgravity Space and Planetary Science Biophysics General Earth and Planetary Sciences Clinostat Gravitation |
| Zdroj: | Advances in space research : the official journal of the Committee on Space Research (COSPAR). 21(8-9) |
| ISSN: | 0273-1177 |
| Popis: | Changes in cellulose and callose content during cell wall regeneration in Brassica oleracea protoplasts have been examined by cytofluorimetry following their exposure to the conditions of the horizontal clinostat (2 r.p.m.) for 10 days. In comparison with controls, cellulose content decreased 4-fold and 28% of the protoplasts failed to resynthesize a wall in the clinorotated sample. The callose content was almost doubled in clinostated cells. Callose synthesis fluctuated in both control and clinorotated protoplasts. The results support the idea that inhibition of cellulose synthesis in protoplasts grown on the clinostat is caused by a change of plasmalemma fluidity and functioning, and also by a disturbance to the state of cytoplasmic calcium under conditions of simulated microgravity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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