C3F gene mutation is involved in the susceptibility to pre-eclampsia
| Autor: | Mohamed Jalloul, Raja Felah, Mohamed Salah Rhim, Ons Kaabia, Mohamed Sakouhi, Sawsen Meddeb, Besma Bel Hadj Jrzad |
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| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Genotype Population Gene mutation Bioinformatics Polymerase Chain Reaction Gastroenterology Gene Frequency Pre-Eclampsia Pregnancy Internal medicine medicine Humans Genetic Predisposition to Disease Prospective Studies Neonatology Allele education Allele frequency Alleles education.field_of_study Polymorphism Genetic Eclampsia business.industry Infant Newborn Obstetrics and Gynecology Complement C3 Exons General Medicine medicine.disease Case-Control Studies Mutation Female Gene polymorphism business |
| Zdroj: | Archives of Gynecology and Obstetrics. 291:1023-1027 |
| ISSN: | 1432-0711 0932-0067 |
| DOI: | 10.1007/s00404-014-3515-y |
| Popis: | The aim of this study was to analyze the functional polymorphism of exon 3 of the gene of complement component C3 (rs 2230199) to identify the potential involvement of the mutated gene C3F in the genesis of pre-eclampsia. It is a comparative case–control study conducted in the university center of maternity and neonatology of Monastir with collaboration of high institute of biotechnology (Tunisia) on a period of 2 years. Two hundred and fifty patients and 96 newborns divided into pre-eclampsia group (150 parturients with pre-eclampsia and 48 newborns) and control group (100 parturients with normal pregnancy and their 48 infants) are taken. Each patient and control were sampled for the phenotypic study and the molecular analysis. The ARMS-PCR (amplification refractory mutation system) was the standard procedure in our study. A simple observation let to distinguish three cases of genotypes: SS, FF and SF. In the control group, 56 % of parturients had the genotype SS, 38 %, the genotype SF and 6 %, FF genotype. In the pre-eclamptic population, SS, SF, and FF genotypes were determined, respectively, 40, 45.30 and 14.60 % of the patients. There is a sharp increase in the frequency of the FF genotype in pre-eclamptic patients compared to controls (14.60 vs. 6 %). The difference was statistically significant (p = 0.01). The frequencies of C3S and alleles C3F determined in controls (respectively, 74 and 26 %) were different from those identified in pre-eclamptic patients (respectively, 62.60 and 37.30 %). This difference was statistically significant (p = 0.005). The C3S and C3F allele frequencies determined in control newborns (respectively, 83.33 and 16.66 %) were slightly different from those identified in newborn issued from pre-eclamptic patients (respectively, 80.2 and 19.79 %), but the difference was not statistically significant (p = 0.67). The gene polymorphism of complement component C3 was significantly associated with the onset of pre-eclampsia. These results should be confirmed by other studies looking at larger scale to consider this gene as a new biomarker with predictive potential therapeutic consequences. |
| Databáze: | OpenAIRE |
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